龙葵碱对人肝癌HepG2细胞N-乙酰基转移酶活性的影响  

Effect of solanine on N-acetyltransferase activity in HepG2 cell

作　　者：高世勇[1,2] 季宇彬[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《中草药》2008年第11期1688-1691,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30400591);黑龙江省自然科学基金资助项目(D2004-13);哈尔滨市青年基金资助项目(2004AFQXJ035)

摘　　要：目的探讨龙葵碱对HepG2细胞N-乙酰基转移酶(NAT)活性的影响。方法采用HPLC方法,以2-氨基芴(2-AF)为底物,以2-AF被NAT乙酰化为2-乙酰氨基芴(2-AAF)的量来反应NAT的活性。结果龙葵碱能显著降低HepG2完整细胞NAT的活性;龙葵碱能够降低HepG2细胞质内NAT的活性,作用具有剂量依赖性。结论龙葵碱通过抑制HepG2细胞NAT的活性发挥细胞毒作用。Objective To explore the effect of solanine on N-acetyhransferase （NAT） activity in HepG2 cell. Methods Employing HPLC, using 2-AF as substrate, taking concentration of 2-AF as concentration of substrate, in intact HepG2 cells and their cytoplasm, taking the speed of 2-AF being acetylated to 2-AFF by NAT as the activity of NAT. Results The results show that solanine could inhibit the activity of NAT in intace HepG2 cell and the cytoplasm. Conclusion Solanine acts to cytotoxicity to HepG2 cell by inhibiting the NAT activity.

关 键 词：龙葵碱 HEPG2细胞 N-乙酰基转移酶(NAT) 

分 类 号：R286.91[医药卫生—中药学]