单核细胞的凝血因子活性观察  

Study on the coagulant activity of human blood monocytes

作　　者：贾晓梅[1] 郑缓[1] 王晶 王逸兰[1] 李家增[2] 陆道培[1] 

机构地区：[1]北京医科大学人民医院血液病研究所,100044 [2]中国医学科学院中国协和医科大学血液学研究所

出　　处：《中华血液学杂志》1997年第9期474-476,共3页Chinese Journal of Hematology

摘　　要：目的：探讨单核细胞的凝血与纤溶活性。方法：将体外培养的28×106单核细胞进行－20℃冷冻，离心（3000r／min，10分钟）后，测定上清液及下层液凝血因子活性，并以2．8×106白细胞上清液作对照。结果：单核细胞上清液有凝血因子Ⅱ、Ⅶ、Ⅸ、Ⅹ、Ⅴ及组织因子活性的表达，并具有特异性。因子（F）XIIIA：Ag为2．1％、FXIIIS：Ag为2．8％。t－PA：A及PAI：A未测出。下层液中凝血因子无特异性表达。结论：单核细胞上清液（含胞膜及胞浆）有凝血因子活性。Objective:To investigate the coagulant and fibrinolytic activities of human blood mono cytes. Methods: Cultured monocytes (2. 8 ×106 cells) were refrigerated at - 20℃ and then thawed and centrifuged. Blood coagulant factors activities in the upper and lower layer of the supernatant were as sayed and compared with that in the supernatant of white blood cells (containing 2. 8 ×106 cells). Results:There were factors Ⅱ. Ⅶ. Ⅸ.Ⅹ. Ⅴ.and tissue factor activities in the supernatant, Ⅹ Ⅲ A: Ag was 2. 1 %, Ⅹ Ⅲ S: Ag: 2. 8%,while t-PA: A and PAI: A were undetectable. In the lower layer there was no specific expression of blood coagulant factors. Conclusion:There were coagulant activities in the super natant containing the membrane and cytoplasma of monocytes.

关 键 词：单核细胞 血液凝固 纤维蛋白溶解 凝血因子 活性 

分 类 号：R331.14[医药卫生—人体生理学]