植物内生放线菌活性物质防治猕猴桃溃疡病  

Control Effect of Kiwifruit Bacterial Canker by Active Metabolites from Plant Endophytic Actinomycetes

作　　者：申哲[1] 黄丽丽[1] 涂璇[2] 程晶晶[1] 康振生[1,3] 

机构地区：[1]西北农林科技大学:植物保护学院,杨凌712100 [2]西北农林科技大学:生命科学学院,杨凌712100 [3]西北农林科技大学陕西省农业分子生物学重点实验室,杨凌712100

出　　处：《中国生物防治》2008年第4期329-334,共6页Chinese Journal of Biological Control

基　　金：教育部“长江学者和创新团队”支持计划(PCSIRT 200558);高等学校学科创新引智计划资助项目(B07049);公益性行业(农业)科研专项(nyhyzx07-055)

摘　　要：以供试内生放线菌发酵液的皿内抑菌活性为指标,得到对猕猴桃细菌性溃疡病菌有抑制活性的菌株76株,其中35株的发酵液对猕猴桃溃疡菌的抑菌圈直径≥20mm,5株对猕猴桃溃疡病菌均具有显著的抑制活性。其中gCLA4菌株发酵液抑菌谱最广,不仅对4种靶标细菌具有抑制活性,还对10种植物病原真菌有较强的抑制活性。通过发酵液制备及大孔吸附树脂D101处理得到gCLA4菌粗提活性物质。田间防治结果表明,gCLA4菌株粗提活性物质100倍稀释液对猕猴桃溃疡病28d的相对防效达64.9%,明显优于化学药剂施那宁。Inhibition-zone-diameter to Pseudomonas syringae pv. acitnidae was taken as a parameter to judge the bioactivities of ferments of endophytic actinomycetes. 76 bioactive strains were screened out from the 242 strains, of which 35 strains showed a bigger（ ≥20mm） inhibition-zone-diameter, in which 5 strains presented an inhibiting activity to the P. syringae pv. actinidae. And ferments of gCLA4 pre- sented a much broader spectrum, which not only inhibit the four bacteria but also inhibit 10 plant pathogenic fungi. By the treatment of macroporous adsorptive resins D101, the crude metabolites in ferments of gCLAd had been gotten. The result of the field test indicated that the active metabolites of gCEA4 had an obvious activity to control P. syringae pv. actinidae, the relative control efficiency could reach to 64.9 % after 28 d.

关 键 词：猕猴桃溃疡病 拮抗内生放线菌 田间防治 大孔吸附树脂D101 

分 类 号：S436.634.12[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]