红海榄根部总蛋白质提取方法的改进和双向电泳体系的建立  

Improvement of protein extraction method from Rhizophora stylosa root and establishment of two-dimensional electrophoresis

作　　者：范吉星[1] 邓用川 黄惜 林栖凤 罗越华[1,2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]海南省耐盐作物生物技术重点实验室,海南海口570228

出　　处：《湖南农业大学学报（自然科学版）》2008年第5期549-553,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：海南省重点科技项目(04103);海口市农业三项科技项目;海南省耐盐作物生物技术重点实验室开放基金

摘　　要：分别采用TCA-丙酮沉淀法、酚法、尿素法、周涵韬法和笔者建立的酚改良法,提取红海榄根部总蛋白质,并进行了SDS-PAGE分析和双向电泳体系的优化.结果表明,酚改良法提取红海榄根部总蛋白质的纯度最高,质量最好,为建立双向电泳分析体系提供了较为理想的蛋白样品,获得了良好的双向电泳图谱.该结果为盐生植物,尤其是盐生木本植物,建立了一套可靠的蛋白提取和双向电泳方法.Several methods of plant tissue protein extraction （TCA-acetone precipitation method, phenol method, urea method, Zhou Han-tao described method, and phenol method improved by the authors） were applied to extract total protein from Rhizophora stylosa root, and then after SDS-PAGE, a subsequent 2-DE analysis was carried out with the optimized conditions for 2-DE . The results indicated that, using the modified phenol method, by the authors more qualified protein with higher purification could be obtained compared with other methods, it also could provide a more suitable protein sample and lead to a better spot separation in the 2-DE analysis. Thus, a set of methods were established for reliable protein extraction and 2-DE analysis for salt-tolerant plants, particularly woody halophytes.

关 键 词：红海榄 蛋白质 双向电泳 

分 类 号：Q946.1[生物学—植物学]