枳楂健胃颗粒质量标准研究  

Quality Standard of Zhizha Jianwei Granules

作　　者：孙延萍[1] 

机构地区：[1]哈尔滨医科大学附属第一医院,哈尔滨市150001

出　　处：《中国药房》2008年第33期2605-2607,共3页China Pharmacy

摘　　要：目的:建立枳楂健胃颗粒的质量标准。方法:采用薄层色谱(TLC)法对方中枳实和山楂进行定性鉴别;采用高效液相色谱(HPLC)法测定橙皮苷的含量:色谱柱为Diamonsil C18(200mm×4.6mm,5μm),流动相为乙腈-0.2%磷酸溶液(20∶80),流速为1.0mL·min-1,柱温为40℃,检测波长为283nm。结果:TLC鉴别方法专属性强、重现性好,阴性对照无干扰。橙皮苷的进样量在0.102～1.122μg范围内与峰面积积分值呈良好的线性关系(r=0.9998);平均回收率为99.23%,RSD=0.20%(n=6)。结论:所建标准可用于枳楂健胃颗粒的质量控制。OBJECTIVE： To establish the quality standard of Zhizha jianwei granules.METHODS： TLC was used for the identification of Fructus Aurantii Immaturus and Fructus Crataegi.HPLC was applied for the determination of Hesperidin by which Diamonsil C18（200 mm× 4.6 mm, 5 μm）chromatographic column was used and the mobile phase was acetonitrile- 0.2% phosphoric acid（20 ： 80）with a flow rate of 1.0 mL · min^- 1.The column temperature was maintained at 40 12 and the detection wavelength was set at 283 nm.RESULTS： TLC method was proved to be specific and reproducible, and free from the inter- ference of the negative control substance .The linear range of Hesperidin was 0.102-1.122μg（r = 0.999 8） and its average recovery rate was 99.28% （RSD = 0.20%, n = 6） . CONCLUSION：The established standard is suitable for the quality control of Zhizha jianwei granules.

关 键 词：枳楂健胃颗粒 薄层色谱法 高效液相色谱法 质量标准 

分 类 号：R283.625[医药卫生—中药学] R927.1[医药卫生—中医学]