HPLC法测定板蓝根滴丸中腺苷的含量  

Determination of Adenosine in Banlangen Dropping Pills by HPLC

作　　者：符敬伟[1] 黄志军[2] 刘树春[3] 张庆伟[1] 王润玲[1] 

机构地区：[1]天津医科大学药学院,天津市300070 [2]天津市长征医院,天津市300120 [3]天津市食品药品监督管理局,天津市300051

出　　处：《中国药房》2008年第33期2607-2608,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱(HPLC)法测定板蓝根滴丸中腺苷含量的方法。方法:色谱柱为Phenomenex-C18(250mm×4.6mm,5μm),流动相为乙腈-水(6∶94),流速为1mL.min-1,检测波长为260nm。结果:腺苷的检测浓度在6.36～31.8μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9995);平均回收率为104.93%,RSD=0.94%(n=6)。结论:本方法准确、快速、简便,可用于板蓝根滴丸的质量控制。OBJECTIVE： To establish an HPLC method for the assay of Adenosine in Banlangen dropping pills. METH- ODS： The sample was determined by HPLC on a Phenomenex - C18 column（250 mm × 4.6 mm, 5μm） .The mobile phase consisted of acetonitrile-water（6 ： 94） at a flow rate of 1 mL · min^-1 and detected at 260 nm.RESULTS： The calibration curve was linear in the range of 6.36-31.8 μg · mL^- 1. The average recovery of sample was 104.93% （RSD = 0.94%, n = 6） . CONCLUSION ：This method is accurate,rapid and simple,and it can be used for the quality control of Banlangen dropping pills.

关 键 词：板蓝根滴丸 腺苷 高效液相色谱法 含量测定 

分 类 号：R283.64[医药卫生—中药学]