丹参多酚酸盐透析液对透析膜生物相容性的影响研究  

Effects of Salvionolic Acids Dialyzate on Biocompatibility of Hemodialysis Polysulfone Membrane

作　　者：黄颜颜[1] 张敏[1] 王娟[1] 王艳[1] 杨亦彬[1] 

机构地区：[1]遵义医学院附属医院肾病科,遵义市563003

出　　处：《中国药房》2008年第33期2615-2617,共3页China Pharmacy

基　　金：贵州省教育厅自然科学研究项目资助课题(黔教科2003215)

摘　　要：目的:研究丹参多酚酸盐透析液对维持性血液透析(MHD)患者外周血中过敏毒素以及C-反应蛋白(CRP)和白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的影响,探讨丹参多酚酸盐透析液是否有助于改善血膜相容性。方法:选择MHD半年以上的患者36例,随机平均分为3组:常规组及丹参多酚酸盐透析液低、高剂量组(分别含丹参多酚酸盐40、80mg·L-1的浓缩A液)。采用膜透析器(均为首次使用),分别于透析前、透析30min及透析结束后(临下机前5min)静脉采血,采用ELISA法比较观察3组C3a、C5a、CRP、IL-6、TNF-α的变化情况。结果:透析30min后,常规组和低剂量组血清C3a、C5a水平较透析前均显著升高;高剂量组较透析前无明显变化。3组IL-6、TNF-α水平在透析前、后均无明显变化。透析结束后,常规组和低剂量组的血清CRP水平较透析前显著升高;高剂量组透析后血清CRP水平较透析前显著下降。结论:一定量的丹参多酚酸盐透析液能减少透析过程中C3a、C5a以及CRP的产生,在一定程度上能改善透析膜的生物相容性,但不能改善单次透析后血清IL-6、TNF-α水平。OBJECTIVE： To study the effects of Salvionolic acids dialyzate on anaphylatoxin, C-reactive protein（CRP）, interleukin- 6（IL 6） and tumor necrosis factor-α（TNF- α） in peripheral blood of patients with maintenance hemodialysis （MHD） and to discuss whether the Salvionolic acids dialyzate could improve the blood - membrane biocompatibility.METHODS： Thirty- six patients undergoing MHD for at least half a year were randomly divided into 3 groups： control group（conventional hemodialysis, n = 12）, low - dose group （dialysate with salvionolic acids 40 mg· L^- 1, n = 12） and high - dose group（dialysate with Salvionolic acids 80 mg · L^-1, n = 12）. Dialyzer of the polysulfone membranes was firstly used in all patients. Patients＇ blood sampling was scheduled before dialysis, 30 minutes after dialysis and at the end of dialysis（5 minutes before withdrawal of dialyzer） for measurement of serum levels of complement（C3a, CSa）,CRP, IL-6 and TNF-α using enzyme linked immunosorbent assay （ELISA） .RESULTS： As compared with before dialysis, the levels of C3a and C5a in both control group and low- dose group were evidently increased at 30 minutes, while the difference in high - dose group was nonsignificant. No significant differences of IL - 6 and TNF- α were found between pre dialysis and post - dialysis in three groups.As compared with before dialysis, the concentration of CRP in control and low- dose groups obviously increased but decreased obviously in high- dose group after dialysis.CONCLUSION： The production of C3a,C5a and CRP in the process of hemodialysis could be inhibited by a certain dose of Salvionolic acids dialyzate, which can improve the biocompatibility of the dialysis - membrane to some degree, but can＇ t improve the levels of IL - 6 and TNF - α after single - time dialysis.

关 键 词：慢性肾衰竭 血液透析 生物相容性 丹参多酚酸盐 

分 类 号：R283.61[医药卫生—中药学] R459.5[医药卫生—中医学]