人外周血T淋巴细胞集落(TL—CFU)培养的研究  

THE STUDIES ON THE COLONY CULTURE OF T-LYMPIIOCYTE IN HUMAN PERIPHERAL BLOOD

作　　者：袁伯伦[1] 乔军[2] 夏光明[1] 魏云松[1] 

机构地区：[1]中日友好医院小儿血液研究室,100029 [2]中日友好医院临床医学研究所免疫室

出　　处：《中国小儿血液》1997年第1期16-19,共4页China Child Blood

摘　　要：本文报道用PHA作为刺激物，采用简化的一步法在甲基纤维素培养基上培养人外周血T淋巴细胞集落（TL一CFU）获得成功，并对TL一CFU培养的适宜条件进行了探讨；同时，对集落组成细胞进行了T淋巴细胞表型分析；在此适宜条件下，对健康成人外周血TL—CFU产率进行了测定。结果显示：（1）培养体系中PHA2ul／ml、20％小牛血清（FCS）、5×10（－5）M2一巯基乙醇、0．8％甲基纤维素、60％RPMI1640、2×105MNC／ml、培养时间七天为TL—CFU培养的适宜条件；（2）集落组成细胞中CD3+细胞占92．5±4．59％、CD4+、CD8+细胞分别为57．83±7．85％及46．66±4．76％、CD4／CD8之比为1．24±0．17；（3）健康成人外周血TL一CFU产率为351．58±21.97／2×105MN。基于上述结果认为，该培养方法操作简便、集落产率高，为研究T淋巴细胞及其亚群提供了条件。This paper shows that one-step culture of human T lymphocyte clonies stimulated by PHA in cellulose medium has been got succesful. The optimal conditions of the culture systems were studied and the colony growth rate of TL-CFU in human peripheral blood was observed in the optimal conditions. The same time, the phenotype of cells in TL-CFU was studied.The findings suggests that: (l ) in the clllture system, the optimal conditions are PHA 2ul/ml, 20%FCS, 5×10-5 M2-ME, 0. 8 % celluose, 60 % RPMI 1640, 5 × 105MNC/ml and 7 days culture; (2 ) the percentages of cells in TL-CFU are CD3+cells 92. 5± 4. 59 %, CD4+cells 57. 83± 7. 85 %and (CD8+cells 46. 66±4. 76%, respectively. The ratio of CD4+cells to CD8+cells is l.24±0. 17; (3) the colony growth rate of TL-CFU is 351. 58± 21. 97 in the peripheral blood of healthy. All these results indicate that the culture method in simple convenient to operate and the growth rate of tL-CFU is higher. This achievement might pare the way for studies on T lymphocytes and subpopulations of them.

关 键 词：T淋巴细胞 T淋巴细胞集落 单克隆抗体 表型 

分 类 号：R392.12[医药卫生—免疫学]