用M13小卫星探针发现猪DNA指纹  

DNA Fingerprinting in Pig Produced by Minisatellite Probe from Bacteriophage M13

作　　者：刘小军[1] 周庆[1] 

机构地区：[1]山西农业大学动物科学系

出　　处：《山西农业大学学报》1997年第3期232-234,共3页

基　　金：山西省青年科学基金

摘　　要：用Ｍ１３ｍｐ１８含基因Ⅲ区的ＣｌａⅠ限制性酶切片段为探针，与猪基因组ＤＮＡ的ＨｉｎｆⅠ限制性酶切产物进行杂交，发现了ＤＮＡ指纹，平均图带数为１７．３１条。说明Ｍ１３ｍｐ１８小卫星序列可作为猪ＤＮＡ指纹图谱分析的探针。Hypervariable DNA fingerprinting in pig was generated by hybridization against pig genomic DNA digesis with HinfⅠusing claⅠrstraction fragments containing gene region Ⅲ of Bactrio-phage M13 as probe.The average bands were 17 31.This results suggested minisatellite sequence from Bacteriophage M13 is suitable as a probe tp genetate pig DNA fingerprinting.

关 键 词：猪 小卫星探针 DNA指纹 

分 类 号：S828.2[农业科学—畜牧学]