梅毒螺旋体47ku抗原的分段表达及抗原表位分析  

Expression of Treponema pallidum 47 ku Fragments and Analysis of Its Epitope

作　　者：郭清顺[1] 李廷栋[1] 熊君辉[1] 周国梁[1] 葛胜祥[1] 李少伟[1] 张军[1] 夏宁邵[1] 

机构地区：[1]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心,生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第6期874-878,共5页Journal of Xiamen University：Natural Science

基　　金：国家863重点项目(2006AA020905);福建省科技重大专项(2004YZ01)资助

摘　　要：通过大肠杆菌表达系统表达了梅毒螺旋体47 ku膜蛋白及其N端和/或C端缺失的重组蛋白共15个,经亲和层析纯化,并通过免疫印迹和酶联免疫实验检测这组蛋白与梅毒病人血清的反应性.结果发现所有包含D结构域的重组蛋白的免疫反应性显著高于不含D结构域的蛋白,提示D结构域中可能包含47 ku膜蛋白上的一个免疫优势表位.Treponema pallidurn 47 ku antigen and its N or C terminal truncated mutants were expressed in E. coli and the recombinant proteins were purified by affinity chromatography. Western blot and ELISA were used to detect the reactivity of these recombinant proteins with the sera of syphilitic patients. The results show that the reactivity of TP47 proteins with domain D are significantly higher than that without domain D,which suggests that there is an immunodominant epitope within domain D of TP47.

关 键 词：梅毒螺旋体 47 ku膜蛋白 免疫反应性 表位 

分 类 号：Q365.1[生物学—遗传学]