酒色着色菌氢酶基因(hydSL)的克隆及分析  

Cloning and Characterization of Hydrogenase Gene hydSL from Chromatium vinosum

作　　者：梁翼[1] 甘礼惠[1] 邬小兵[1] 徐惠娟[1] 胡忠[1] 龙敏南[1] 

机构地区：[1]厦门大学生命科学学院,能源研究院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第6期907-911,共5页Journal of Xiamen University：Natural Science

基　　金：国家863计划项目(2006AA05Z111);福建省自然科学基金(C0410002);福建省科技项目(2006H0091,2005I016);厦门大学新世纪优秀人才计划项目资助

摘　　要：光合细菌酒色着色菌(Chromatium vinosum)至少含有一种膜结合态氢酶(623、2 ku)和一种可溶性氢酶(52、21.5ku).其膜结合态氢酶是一种催化吸氢的氢酶,而可溶性氢酶则是一种催化放氢的氢酶.本研究分离提纯了C.vinosum膜结合态氢酶,并对其大、小亚基N-末端氨基酸序列进行测定;根椐氢酶亚基N-末端氨基酸序列及氢酶保守序列设计引物,通过PCR扩增分别获得了1.1和3.5 kb的DNA片段.对该片段进行克隆和序列分析,结果表明该基因编码氢酶HydSL,其结构不同于典型的氢酶基因结构,在氢酶大、小亚基基因间插入了ISP基因.The photosynthetic bacteria Chromatium vinosum contained at least a membrane-bound hydrogenase and a soluble hydrogenase. A membrane-bound hydrogenase from C. vinosum was purified and identified to consist of two subunits （62,32 ku）. According to the N-terminal sequences of the large subunit： N＇-SEEIVVDPITRIEGH and the small subunit.. N＇-SEQARRPSVIWLSFQE,and the conserved motif of NiFe-hydrogenase,two pairs of primers were synthesized and applied for PCR to amplify the fragment encoding hydrogenase. The sequence analysis results showed that the hydSL contained the unusual gene organization which existed one ISP gene between the small and large subunit. It was suggested that there are more than one membrane-bound hydrogense existed in C. vinosum.

关 键 词：酒色着色菌 氢酶 基因克隆 

分 类 号：Q936[生物学—微生物学]