苎麻UDPGDH基因的cDNA克隆及表达分析  

Cloning and Expression of UDP-glucose Dehydrogenase (UDPGDH) cDNA in Ramie (Boehmeria nivea (Linn.) Gaud.)

作　　者：刘峰[1] 黄妤[2] 郭清泉[1] 张学文[2] 李良勇 邓晶[2] 谢玲玲[1] 

机构地区：[1]湖南农业大学苎麻研究所,长沙410128 [2]湖南农业大学生物科学技术学院,长沙410128 [3]长沙市烟草公司浏阳市营销部,湖南浏阳410300

出　　处：《中国农业科学》2008年第11期3542-3548,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30571186)

摘　　要：【目的】分离苎麻尿苷二磷酸葡萄糖脱氢酶(UDPGDH)基因。【方法】通过简并引物RT-PCR结合RACE技术和半定量RT-PCR。【结果】成功克隆苎麻Boehmeria nivea(Linn.)Gaud.尿苷二磷酸葡萄糖脱氢酶(UDPGDH)基因cDNA序列,序列长1837bp,编码一480个氨基酸的蛋白质(GenBank No.EF178294)。半定量RT-PCR分析显示,UDPGDH在苎麻根、皮、茎和叶组织中均有表达,其表达量为茎>皮>叶>根,相对表达量依次为0.7075,0.3051,0.2651,0.1490。【结论】苎麻UDPGDH与其它植物相应序列具有较高同源性,在苎麻茎中有较高的表达。[Objective] This research attempted to isolate UDP-glucose dehydrogenase gene in ramie （Boehmeria nivea）. [Method] Degenerate primer was designed to amplify a fragment of UDPGDH gene so as to obtain the full length cDNA using RACE. The expression of UDPGDH gene in different tissues of ramie was analyzed by semi-quantitative RT-PCR. [Result] Full length ramie UDPGDH cDNA of 1 837 bp, encoding protein of 480 amino acids in reading frame （GenBank No. EF178294） was cloned. The expression of UDPGDH was found in leaf, stem, root and phloem in ramie. The expression levels in different tissues from stem, phloem, leaf, and root ranking high to low, with the relative content of 0.7075, 0.3051, 0.2651, 0.1490, respectively. [ Conclusion ]Ramie （Boehmeria nivea） UDPGDH cDNA is homologous with other plants with the highest expression level in stem.

关 键 词：苎麻 尿苷二磷酸葡萄糖脱氢酶(UDPGDH)基因 CDNA克隆 表达分析 

分 类 号：S563.1[农业科学—作物学]