应用ISSR对25个小菊品种进行遗传多样性分析及指纹图谱构建  

Genetic Diversity and Construction of Fingerprinting of Chrysanthemum Cultivars by ISSR Markers

作　　者：缪恒彬[1] 陈发棣[1] 赵宏波[1] 房伟民[1] 石丽敏[1,2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]浙江省东阳市玉米研究所,浙江东阳322105

出　　处：《中国农业科学》2008年第11期3735-3740,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30400308);上海市农委重点攻关项目[农科攻字(2004)第3-1]

摘　　要：【目的】揭示选育出的菊花新品种遗传多样性并建立ISSR指纹图谱,为品种知识产权保护提供依据。【方法】选用21个ISSR引物,对新选育的25个小菊品种的基因组DNA进行随机引物PCR扩增。【结果】共获得122条扩增带,其中多态性谱带114条,占扩增谱带数的93.4%;品种间相似系数变幅在0.282～0.757;平均有效等位基因数为1.6390,平均Nei’s基因多样性指数为0.3620,平均Shannon信息指数为0.5323;在21个引物中,引物ISSR35扩增的谱带可把25个品种完全区分开。【结论】ISSR标记技术能较好地从分子水平揭示出菊花品种的遗传多样性,并能有效应用于菊花品种指纹图谱的构建。[Objective] This study was carried out in order to establish the ISSR fingerprinting of new Chrysanthemum cultivars which is applicable in cultivar identification and breeder＇s fights protection of chrysanthemum. [Method] The genomic DNA was used as template and random primers were used to develop fingerprinting of 25 cultivars of Chrysanthemum morifolium Ramat. by ISSR-PCR. [Result] Of the 77 primers screened, 21 primers could generate 122 fragments, 93.4% of which were polymorphic ones. The similarity coefficient of cultivars was between 0.282-0.757. The average value of effective number of alleles, Nei＇s gene diversity and Shannon＇s information index were 1.6390, 0.3620, and 0,5323, respectively. The 25 cultivars could be identified and discriminated efficiently by primer ISSR35. [Conclusion] The results showed that ISSR approach is efficient in more accurate information on genetic relationship, diversity and establishment of fingerprinting of Chrysanthemum cultivars.

关 键 词：菊花 ISSR-PCR 遗传多样性 指纹图谱 

分 类 号：S682.11[农业科学—观赏园艺]