Protein surface recognition of the novel tetra-carboxylphenyl calix[4]arene to cytochrome c  

作　　者：Wen Ting An Yong Jiao Xiao Hua Sun Chuan Dong Shao Min Shuang Ping Fang Xia Man Shing Wong 

机构地区：[1]Research Center of Environmental Science and Engineering, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China [2]Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hongkong S.A.R., China

出　　处：《Chinese Chemical Letters》2008年第11期1341-1344,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.90813018);the Youth Foundation of Shanxi Province (No.2006021009);as well as the Youth Scientific and Technical Foundation of Shanxi University (Nos.2006007 and 2007112).

摘　　要：The interaction of the novel tetra-carboxylphenyl calix[4]arene （TCPC） with the bovine heart cytochrome c （Cc） was first investigated by fluorescence spectroscopy and molecular modeling methods. The formation of a stable 1：1 complex was monitored by fluorescence titration, and its binding constant is 1.916 ×10^7 L mol^-1. Molecular modeling reveals the recognition mechanism of TCPC to the Cc surface, that is, the electrostatic interaction drives TCPC to the Cc surface, and the van der Waals interaction orientates TCPC parallel to the cleft of Cc.The interaction of the novel tetra-carboxylphenyl calix[4]arene （TCPC） with the bovine heart cytochrome c （Cc） was first investigated by fluorescence spectroscopy and molecular modeling methods. The formation of a stable 1：1 complex was monitored by fluorescence titration, and its binding constant is 1.916 ×10^7 L mol^-1. Molecular modeling reveals the recognition mechanism of TCPC to the Cc surface, that is, the electrostatic interaction drives TCPC to the Cc surface, and the van der Waals interaction orientates TCPC parallel to the cleft of Cc.

关 键 词：Tetra-carboxylphenyl arene Cytochromc c Protein surface recognition Fluorescence spectroscopy Molecular modeling 

分 类 号：Q51[生物学—生物化学]