α-葡萄糖苷酶工程菌发酵条件的研究  

Study on culture conditions of engineered strain of α-glucosidase

作　　者：于岚[1] 张云开[2] 覃拥灵[2] 陈桂光[2] 梁智群[2] 

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000 [2]广西大学生命科学与技术学院,广西南宁530004

出　　处：《食品工业科技》2008年第11期60-62,共3页Science and Technology of Food Industry

基　　金：国家863计划资助项目(2006AA10Z339)

摘　　要：工程菌DHS-2携带来自黑曲霉(Aspergillus niger)M-1菌株的高转苷活性α-葡萄糖苷酶基因。对DHS-2的发酵条件进行了优化研究,使其α-葡萄糖苷酶活力由178.53U/mL提高到618.75U/mL,提高了2.5倍。质粒稳定性研究表明,DHS-2在传代100代以内,质粒的保留率为82%以上,表现出较好的稳定性。The recombinant E. coli DHS-2 carried α-glucosidase gene from A. niger M-1.The condition for expressions of recombinant α-glucosidase was studied. On the optimum condition,the α-glucosidase activity of DHS-2 was increased from 178.53U/mL to 618.75U/mL, increased by 2.5 folds. 82.% cells still hold the recombinant plasmid after cultivation in LB broth at 37℃ for 100 generation without antibiotic selection.

关 键 词：Α-葡萄糖苷酶 工程菌DHS-2 培养条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]