肠内免疫营养和生态营养对创伤后大鼠肠屏障功能的影响  

Effects of Enteral Immunonutrition and Econutrition on Intestinal Mucosa Barrier Function in Wounded Rats

作　　者：余利坚[1] 徐亮[2] 姚晖[2] 魏仁志[2] 綦晓龙[2] 

机构地区：[1]泸州市人民医院肛肠科,泸州646000 [2]泸州医学院附属医院胃肠外科,泸州646000

出　　处：《中国普外基础与临床杂志》2008年第11期794-798,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的研究肠内免疫营养和生态营养对创伤后机体肠道屏障功能的影响。方法将40只Wistar大鼠按随机数字表法均分为4组,即对照组、肠内普通营养组、肠内免疫营养组及肠内生态营养组。于胃造瘘术后早期分别给予普通饲料、肠内普通营养剂、肠内免疫营养剂和肠内生态营养剂,共7d,观察小肠黏膜形态和黏膜IgA+、CD3+、CD4+和CD8+细胞数量。结果小肠绒毛高度、肠腺隐窝深度、黏膜厚度(除肠内普通营养组)以及绒毛表面积在肠内普通营养组、肠内免疫营养组和肠内生态营养组均优于对照组(P<0.05),各肠内营养组之间的差异无统计学意义(P>0.05)。小肠黏膜IgA+、CD3+、CD4+及CD8+细胞数对照组大鼠低于各肠内营养组(P<0.05),而肠内普通营养组又低于肠内免疫营养组和肠内生态营养组(P<0.05)。结论肠内免疫营养和生态营养能较好地改善创伤后大鼠的小肠机械屏障功能,促进小肠黏膜屏障功能的恢复,增强其肠道免疫功能。Objective To study effects of enteral immunonutrition and econutrition on intestinal mucosa barrier function in wounded rats. Methods Forty Wistar rats were randomly divided into four groups, with ten rats in each group [ie. control group, enteral nutrition （EN） group, enteral immunonutrition （EIN） group and enteral eeonutrition （EEN） group]. After gastrostomy, rats in each group were treated with the isoealorie and isonitrogenous nutritional formulas for 7 days, respectively. The morphology of ileum membrane was studied, and the quantities of IgA^＋ , CD3^＋ , CD4^＋ and CD8^＋ ceils （each HP） of ileum membrane were determined. Results The villus height, crypt depth, mucosal thickness （except EN group） and villus surface area of ileum were increased in EN, EIN and EEN group compared with control group （P〈0.05）, but there was no significant difference among the former three groups （P〉O. 05）. The numbers of IgA^＋ , CD3^＋ , CD4^＋ and CD8^＋ cells were increased in EN, EIN and EEN group compared with control group （P〈0.05）, and those numbers in EN group were lower than those in EIN and EEN group （P〈0.05）. Conclusion EIN and EEN may improve intestine mechanical barrier function and promote restoration of small intestine mucous membrane barrier function in rats. EIN and EEN also improve intestine immune barrier function and strengthen its immune function.

关 键 词：肠内营养 免疫营养 生态营养 动物实验 WISTAR大鼠 

分 类 号：R459.3[医药卫生—治疗学]