家蚕血液对BmE-SWU1细胞凋亡的抑制作用  

Inhibition of BmE-SWU1 cell apoptosis by silkworm hemolymph

作　　者：潘敏慧[1] 邓为科[1] 陈默[1] 张金叶[1] 杜娟[1] 鲁成[1] 

机构地区：[1]西南大学蚕学与系统生物学研究所,重庆400716

出　　处：《动物学报》2008年第5期861-866,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金项目(No.30771630);重庆市自然科学基金项目(No.CSTC;2007BB1337)~~

摘　　要：以家蚕胚胎细胞系BmE-SWU1细胞为体外模型,用不同浓度的放线菌素D处理家蚕BmE-SWU1细胞进行家蚕细胞凋亡研究。结果表明:放线菌素D诱导家蚕细胞凋亡的作用呈时间、剂量依赖性。分别用浓度为0、50、100和200ng/ml的放线菌素D处理BmE-SWU1细胞12h后,凋亡峰所占比例分别为1.82%、1.26%、8.21%和12.31%。当放线菌素D的浓度为100ng/ml时,诱导家蚕BmE-SWU1细胞凋亡的效果显著;家蚕血液对放线菌素D诱导的家蚕BmE-SWU1细胞凋亡具有明显的抑制作用。It is important to reveal the mechanisms of developmental biology and genie control for absolutely metamorphic insects, as well as to research sericulture science, that apoptosis of Bombyx mori was researched with BmE-SWU1 cells treated with various concentration of Actinomycin D. After BmE-SWU1 cells were treated with various concentration of Actinomycin D, the effects of apoptosis depend on time and dose in BmE-SWU1 cells and the apoptosis is very significant when cells were treated with Actinomycin D 12 h at 100 ng/ml. Silkworm hemolymph has significant inhibition to BmE-SWU1 cells apoptosis induced by actinomycin D

关 键 词：家蚕 家蚕血液 BmE-SWU1细胞系 细胞凋亡 凋亡抑制 

分 类 号：Q96[生物学—昆虫学]