许旺细胞植入去细胞异体神经修复大鼠面神经缺损  

Repair of facial nerve defects by using acellular nerve allografts implanted with Schwann cells in rats

作　　者：朱国臣[1] 肖大江[1] 黄红宇[2] 袁渊[1] 吴四海[1] 赵新[1] 

机构地区：[1]无锡市第二人民医院耳鼻咽喉科, 214002 [2]无锡市第二人民医院中心实验室, 214002

出　　处：《中华创伤杂志》2008年第11期897-899,共3页Chinese Journal of Trauma

摘　　要：目的观察体外分离、培养的许旺细胞对去细胞异体神经修复面神经长距离缺损的促进作用。方法30只大鼠随机分成A组、B组和C组（每组10只），制成面神经缺损12mm模型，分别以去细胞异体神经联合许旺细胞、去细胞异体神经和自体神经移植修复。术后5个月分别行神经电生理、再生神经形态及数量等检测。结果A组神经肌肉动作电位的波幅恢复率（术侧／健侧）为（35．8±2．5）％，潜伏期恢复率（健侧／术侧）为（65．8±2．9）％；神经移植段再生轴突数为（1570±188）个，髓鞘厚度为（0．383±0．031）μm。A组的各项指标优于B组（P〈0．05），与C组差异无统计学意义（P〉0．05）。结论许旺细胞植入去细胞异体神经可促进面神经长距离缺损的修复。Objective To observe the effects of in vitro isolated Schwann cells co-cultured with chemically acellular nerve allografls on improving repair of large facial nerve defects. Methods A total of 30 Wistar rats were equally randomized into three groups, ie, experimental group, allograft group and autograft group. Nerve defect of 12 mm in length was made in the left inferior buecal branch of facial nerve and repaired with aeellnlar nerve allograft implanted with Sehwann cells, acellnlar nerve allograft and fresh tibial nerve autograft respectively. At the 5th month postoperatively, the function and morpholo- gy of the regenerated nerves were observed by electrophysiological method, methylene blue staining and transmission electron microscope. Results In experimental group, the recovery rate （operation side/ normal side） of amplitude of nerve-muscle action potential was （35.8 ±2.5 ）%, the lantency recovery rate （ normal side/operation side） （65.8 ±2.9） %, the number of the regenerated axon 1 570 ± 188 and the myelin thickness （0.383 ± 0.031 ） μm. The results in the experimental group were significantly superior to those in the acellular nerve allograft group （ P 〈 0.05）, with similar results to fresh nerve autograft group （ P 〉 0.05）. Conclusion Transplantation of Schwann cells in acellular nerve allograft can improve repair of large facial nerve defects.

关 键 词：创伤和损伤 面神经 神经再生 许旺细胞 移植 同种 

分 类 号：R651.3[医药卫生—外科学] R779.64[医药卫生—临床医学]