Molecular-Cytological Identification and Chromosome Behavior Analysis of Telotetrasomic in Rice  

作　　者：GONG Zhi-yun GAO Qing-song Yu Heng-xiu YI Chuan-deng Gu Ming-hong 

机构地区：[1]Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China

出　　处：《Rice science》2008年第3期161-165,共5页水稻科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30600345, No. 30770131 and No. 30771210).

摘　　要：From the progenies of a telotrisomic of chromosome 9 short arm of an indica rice variety, Zhongxian 3037, a phenotypical variant was selected. The variant plant had rolled leaves, dispersed plant type, as well as a low seed-setting rate. Cytological and molecular cytological investigations revealed two extra chromosomes, which were the shortest in somatic cells of the variant. Fluorescent in situ hybridization （FISH） analysis using a rice centromere specific DNA （RCS2） and a DNA sequence specific for chromosome 9 on premetaphase and pachytene chromosomes showed that these two chromosomes were the short arms of chromosome 9. That is to say, the variant was a telotetrasomic of chromosome 9. Among the 25 pachytene cells, the two telosomic chromosomes paired each other to form a bivalent and didn＇t pair with other normal chromosome 9 as multivalents in 96% cells. However, the bivalent was easy to disassociate in advance.From the progenies of a telotrisomic of chromosome 9 short arm of an indica rice variety, Zhongxian 3037, a phenotypical variant was selected. The variant plant had rolled leaves, dispersed plant type, as well as a low seed-setting rate. Cytological and molecular cytological investigations revealed two extra chromosomes, which were the shortest in somatic cells of the variant. Fluorescent in situ hybridization （FISH） analysis using a rice centromere specific DNA （RCS2） and a DNA sequence specific for chromosome 9 on premetaphase and pachytene chromosomes showed that these two chromosomes were the short arms of chromosome 9. That is to say, the variant was a telotetrasomic of chromosome 9. Among the 25 pachytene cells, the two telosomic chromosomes paired each other to form a bivalent and didn＇t pair with other normal chromosome 9 as multivalents in 96% cells. However, the bivalent was easy to disassociate in advance.

关 键 词：RICE telotetrasomic MEIOSIS fluorescent in situ hybridization molecular cytology chromosome behavior 

分 类 号：S511[农业科学—作物学] S1