Over-Expression of ICE1 Gene in Transgenic Rice Improves Cold Tolerance  

作　　者：XIANG Dian-jun Hu Xiang-yang ZHANG Yu YIN Kui-de 

机构地区：[1]School of Life Science and Biotechnology, Heilongjiang August First Land Reclamation University, Daqing 163319, China [2]Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China [3]Heilongjiang Agricultural Economy Professional College, Mudanjiang 157032, China

出　　处：《Rice science》2008年第3期173-178,共6页水稻科学（英文版）

基　　金：supported by a project grant from the Education Department of Heilongjiang Province, China (Grant No. 11511248).

摘　　要：ICE1, an Arabidopsis thaliana transcription factor gene, was cloned by RT-PCR and successfully transformed into rice variety Kenjiandao 10 by the Agrobacterium-mediated transformation method. PCR amplification and Southern blot analysis indicated that ICE1 had been integrated into rice genome. Compared with the non-transgenic plants, the transgenic plants exhibited high resistance to hygromycin B and were consistent with the Mendelian inheritance of a single copy of the transgenic ICE1. Under the low temperature stress, the transgenic plants showed the lower mortality rate and the increased proline content. These results suggest that the Arabidopsis ICE1 is functional in rice and the over-expression of ICE1 improves the tolerance to cold stress in rice.

关 键 词：Arabidopsis thaliana transcription factor gene rice genetic transformation cold tolerance proline content 

分 类 号：S511[农业科学—作物学] S18