人H-FABP单克隆抗体制备和纯化及其特性鉴定  

Preparation of monoclonal antibady of human H-FABP

作　　者：许淑芬[1] 侯巍 谭岩[1] 杨述红[3] 车媛媛[1] 王智昊[1] 

机构地区：[1]吉林大学第一医院,吉林长春130021 [2]解放军461医院 [3]空军航空大学门诊部

出　　处：《中国实验诊断学》2008年第11期1343-1346,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技发展计划项目(编号:20030450)

摘　　要：目的制备人心肌型脂肪酸结合蛋白(Heart type fatty acid-binding protein;H-FABP)单克隆抗体。方法(1)应用淋巴细胞杂交瘤技术,将重组人H-FABP腹腔注射免疫BALB/C小鼠。(2)将鼠脾细胞与鼠骨髓瘤细胞(SP2/0)融合、培养。通过HAT和间接ELISA法筛选、鉴定制备稳定分泌抗人H-FABP单克隆抗体的杂交瘤细胞株。(3)利用Protein G Sepharose 4 Fast Flow亲合纯化体内诱生法制备的腹水抗体,并对抗体特性进行鉴定。结果得到7株特异性识别人H-FABP单克隆抗体,并纯化两株亲合力高、空间位点远的单克隆抗体。结论成功制备了稳定分泌抗人H-FABP单克隆抗体的杂交瘤细胞株。Objective Objeetive：Preparition of monoelone antibody to human heart type fatty acid-binding protein. Methods 1）The BALB/C mouse was immuned with intraperitoneal by recombinant humen H-FABP. 2）The spleen cells and myeloma cells of the mouse were fused and cultivated, then monoclonal antibody of human H-FABP was selected. 3）The antibody was purificated by Protein G Sepharose 4 Fast Flow affinity ehromatograph and it was identified. Results 1）Two strains monoclonal antibody whieh lind high affinities and far space sites were gotten and purified. Conclusion The monoclonal antibody of human H-FABP was suecessfully prepareted.

关 键 词：人心肌型脂肪酸结合蛋白 单克隆抗体 ELISA 

分 类 号：R392.11[医药卫生—免疫学]