高效液相色谱法测定双黄连注射剂中黄芩苷的方法分析  

Method Analysis of Determination of Baicalin in Shuanghuanglian Injection by HPLC

作　　者：翟丽[1] 段漓童[1] 郭瑞华[1] 吴来娣[1] 

机构地区：[1]华北煤炭医学院,河北唐山063000

出　　处：《时珍国医国药》2008年第11期2708-2709,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省科研与发展第二批指导计划项目(No.05276460)

摘　　要：目的建立以高效液相色谱（HPLC）法测定双黄连注射剂中组分含量的方法。方法以甲醇-水（10％的磷酸溶液调节pH至2．7）为流动相，流速为1．0ml／min，检测波长为326nm，在Diamonsil（钻石）C18（5μm，4．6mm×150mm）色谱柱上洗脱，外标法定量，黄芩苷含量可测定。结果黄芩苷在5．1～81．6μg/ml范围内呈良好的线性关系，r=0．9998，平均加样回收率为100．34％，RSD=0．82％（n=5）。结论该方法简便，灵敏，重复性好，结果准确，精密度较高，线性关系良好，回收率也较高，测定结果显示12h内黄芩苷稳定，适合于双黄连注射剂的质量控制。Objective To develop an HPLC method for the determination of the components in Shuanghuanglian. Methods The HPLC column was Diamonsil C18 （5 μm, 150 mm ×4.6 mm）. A gradient methanolwater （pH2.7, adjusted by 10% phosphate solution） solvent was used as mobile phase, with a flow rate of 1.0ml/min, and the detection wavelength was 326 nm. Results - Baicalin had a good linear relationship within the range of 5.1 - 81.6μg/ml ,r = 0.9998, and the average recovery was 100.34 % , with the RSD = 0.82 % （ n = 5 ）. Conclusion The method is simple, accurate, highly sensitive, and reproducible, with a high recovery and good linear within the measure limitation. Under the measure condition, baiealin is stable within 12 h, which makes it suitable for the quality control of Shuanghuanglian injection.

关 键 词：双黄连注射剂 黄芩苷 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]