2005—2006年流行的致病性鹅新城疫病毒的分子流行病学特征  

Molecular epidemiologic investigation of Newcastle disease viruses that caused clinical disease in geese during 2005 and 2006

作　　者：张俊涛[1] 万洪全[1] 王小波[1] 吴力力[1] 宋红芹[1] 李书胜[1] 陈露[1] 王宝安[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2008年第10期1154-1157,共4页Chinese Journal of Veterinary Science

基　　金：江苏省青年创新人才基金资助项目(BK2003403);江苏省教育厅自然科学基金资助项目(03KJB230158)

摘　　要：为了对能导致鹅临诊疾病的新城疫病毒（Newcastle disease virus，NDV）进行监测，2005--2006年从江苏、安徽2省不同地区的发病鹅群分离、鉴定了10株NDV，并对其中8株病毒的部分生物学特性及分子生物学特征进行了研究。鸡胚平均死亡时间（MDT）、1日龄雏鸡脑内接种致病指数（ICPI）测定结果表明8株病毒皆为NDV强毒株。对融合蛋白（F）和血凝素-神经氨酸酶（HN）基因序列的分析发现，8株病毒中有7株病毒在基因型分类上属于基因Ⅶd亚型，并与1997—2001年间流行的对鹅具有高度致病性的NDV高度同源（95．5％～99．8％），而且这7株病毒的F、HN蛋白具有出现于2001年以前流行的致病性鹅NDV的绝大部分特征性氨基酸，进一步说明这些特征性氨基酸非常保守，可以作为对鹅具有高度致病性的NDV的分子标志。Since the first report in 1997, the clinical disease in the geese induced by virulent Newcastle disease virus （NDV） has been prevailing in some South-eastern provinces and thus necessitating a continuous surveillance on the viruses. Eight NDVs isolated from diseased geese during the years 2005 and 2006 were analyzed for the molecular epidemiology in the present studies. All of the 8 isolates were identified to be velogenic viruses based on the MDT and ICPI values and the characteristic motif 112R-R-Q-K-R Fl17 at the cleavage site of the fusion （F） proteins. Upon performing sequence analysis of the F protein and hemagglutinin-neuraminidase （HN） genes. 7 of the 8 viruses were determined phylogenetically to be very close to those circulated in the geese during 1997 to 2001,with the remaining one being highly homologous to the reference strain,F48ES. It is of more interest for us to find that these 7 isolates share 12 out of the 13 characteristic amino acids found in the viruses isolated before 2001 ,which highlights the stability of these residues and the potential links between these residues and the virulence of NDVs in the geese. Our results here further suggest that these residues can be used as molecular markers for NDVs to naturally induce clinical disease in the geese.

关 键 词：鹅 新城疫病毒 融合蛋白 血凝素-神经氨酸酶 

分 类 号：S852.65[农业科学—基础兽医学]