不同剂量小鹅瘟病毒VP3基因疫苗肌肉注射诱导小鼠细胞免疫  

Cellular immune responses of BALB/c mice induced by GPV-VP3 DNA vaccine immunized intramuscular injection with different dose

作　　者：卢菲[1] 程安春[1,2] 汪铭书[1,2] 韩新锋[1] 黎敏[1] 车茜[1] 刘晓东[1] 陈孝跃[1,2] 

机构地区：[1]四川农业大学动物医学院禽病防治研究中心,四川雅安625014 [2]四川省动物疫病与人类健康重点实验室,四川雅安625014

出　　处：《中国兽医学报》2008年第10期1158-1161,共4页Chinese Journal of Veterinary Science

基　　金：国家科技攻关重大项目(2004BA901A03);教育部"新世纪优秀人才支持计划"项目(NCET-04-0906);四川省重大基础研究项目(05JY029-109);四川省重点建设学科项目(SZD0418)

摘　　要：将小鹅瘟(Gosling plagne,GP)VP3基因疫苗(pcDNA-GPV-VP3)分别按每只50、100、200μg肌肉注射免疫BALB/c小鼠,以pcDNA3.1(+)和生理盐水为对照,于免疫后7、14、21、28、35、63、105d采血用淋巴细胞转化实验(MTT法)和流式细胞仪(FACS)分别检测小鼠外周血T淋巴细胞转化效果和CD4+、CD8+T淋巴细胞动态变化。结果表明,pcDNA-GPV-VP3免疫小鼠能够诱导机体产生良好的细胞免疫应答。50、100μg pcDNA-GPV-VP3免疫后小鼠外周血T淋巴细胞对ConA刺激的反应显著或极显著高于200μg组、空载体和生理盐水对照组,且以100μg组最优,而200μg组的转化效果比空载体组差;100μg pcDNA-GPV-VP3免疫小鼠后所诱导的CD4+T淋巴细胞免疫功能最强,50μg组次之;50μg pcDNA-GPV-VP3免疫小鼠后所诱导的CD8+T淋巴细胞免疫功能最强,100μg组次之。BALB/c mice were immunized with 50,100,200 ug of pcDNA-GPV-VP3 DNA vaccine respectively by the route of intramuscular injection, pcDNA3. 1 （＋） and physiological saline as controls. Collected the peripheral blood samples at 7,14,21,28,35,63 and 105 d after immunizaton. T lymphocyte proliferation test （MTT） was used to detect the proliferation of T lymphocytes cell,Fluorescence activated cell sorter （FACS） was used to detect the number of CD4^ ＋ ,CD8^＋ cells in peripheral blood lymphocytes. Results：pcDNA-GPV VP3 DNA vaccine induced good cellular immune responses. ①ConA response of T-lymphocytes in blood was significant （P〈0. 05） or highly significant（P〈0.01） differences between 50,100 ug groups and 200 ug group, controls after vaccination. 100 ug group was the best one and the immune response of the 200 ug group was worse than pcDNA3.1（＋）. ②100 ug group in duced best cellular immune response of CD4^＋ T cells,50 ug group was the better one. ③50 ug group induced best cellular immune response of CD8^＋ T cells, 100 ug group was the better one.

关 键 词：小鹅瘟病毒VP3基因疫苗 肌肉注射 小鼠 细胞免疫 

分 类 号：S852.65[农业科学—基础兽医学]