番鸭呼肠孤病毒弱毒株在免疫番鸭体内的分布及排毒规律  

Distribution and excretion of muscovy duck reovirus attenuated strain in vaccinated ducklings

作　　者：林锋强[1] 陈仕龙[1] 胡奇林[1] 程晓霞[1] 王劭[1] 朱小丽[1] 欧阳岁东[1] 陈少莺[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350003

出　　处：《中国兽医学报》2008年第11期1259-1261,共3页Chinese Journal of Veterinary Science

基　　金：福建省自然科学基金资助项目(2008J0061);福建省科技重大专项资助项目(2006NZ0003-2)

摘　　要：番鸭呼肠孤病毒(Muscovy duck reovirus,DRV)B37弱毒株经肌内免疫1日龄雏鸭,应用RT-PCR检测病毒核酸,以阐明病毒在体内分布及排毒规律。结果表明,B37株免疫雏鸭后4h,即可在血液、心、肝、脾组织中检出DRV RNA;接种后8h,血液、心、肝、脾、肺、肾、胰腺均可检测到DRV RNA;接种后3d,喉头和泄殖腔棉拭子可检出DRV RNA;接种后14d,喉头和泄殖腔棉拭子已不能检出DRV RNA;接种后28d,肺、肾和胰腺均已不能检出DRV RNA;接种后42d,血和心脏已不能检出DRV RNA;接种后49d,所有组织均已不能检出DRV RNA。因此,DRV弱毒在血液、心脏中分布时间为接种后4h～35d,在肝、脾组织中分布时间为4h～42d;肺、肾和胰腺的分布时间为8h～25d;向外界排毒时间为3～14d。One-day-old muscovy ducks were immunized with muscovy duck reovirus attenuated strain by the route of muscle injection, then the viruses in blood, heart, liver, spleen, lung, kidney, pancreas, larynx and cloaca were detected by RT-PCR to explore the distribution and excretion in the vaccinated ducklings. The results indicated that the tissue of blood, heart, liver and spleen were positive 4 h post vaccinated（PV） ; lung, kidney and pancreas were positive 8 h post vaccinated;larynx and cloaca were positive 3 d post vaccinated but were negative 14 d post vaccinat- ed;kidney and pancreas were negative 28 d post vaccinated;blood and heart were negative 42 d post vaccinated;all tissues were negative 49 d post vaccinated. Above all, the sustaining time of muscovy duck reovirus attenuated strain was 4 h-35 d in blood and heart,4 h-42 d in liver and spleen;8 h-25 d in lung,kidney and pancreas. The excretion time was 3-14 d.

关 键 词：RT—PCR 番鸭呼肠孤病毒 弱毒株 分布 排毒 

分 类 号：S852.65[农业科学—基础兽医学]