盐酸多西环素在猪体内的药物动力学及其残留  

Pharmacokinetics and residues of doxycycline hydrochloride in pigs

作　　者：王金花[1] 操继跃[1] 翟克影[1] 李欣[1] 曾令高 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]武汉蔡甸区畜牧局,湖北武汉430100

出　　处：《中国兽医学报》2008年第11期1313-1316,1320,共5页Chinese Journal of Veterinary Science

基　　金：盐酸多西环素注射液兽药地方标准升国家标准资助项目

摘　　要：试验建立了反相高效液相色谱(RT-HPLC)法测定盐酸多西环素的浓度,探讨了盐酸多西环素在猪体内的药物动力学和残留特征。结果表明,盐酸多西环素以2.5mg/kg单剂量肌内注射给猪(n=6),药物动力学模型符合有吸收一室模型,药物动力学参数:吸收半衰期(t1/2ka)、消除半衰期(t1/2ke)为(0.400±0.312)h、(9.530±0.956)h,药时曲线下面积(AUC)为(44.414±4.123)mg·h·L-1,最大血药浓度(Cmax)为(2.811±0.136)mg/L,达峰时间(Tp)为(1.910±0.213)h。另外,以相同剂量肌内注射给猪(n=6),每天1次,连续给药4d后,在不同时间测定盐酸多西环素在猪的肌肉、肝脏、肾脏、皮肤和脂肪中的残留量。在给药后16d,盐酸多西环素在各组织均能检测到,且残留均低于残留限量。盐酸多西环素注射液在猪体内消除缓慢,残留期较长,建议休药期不低于16d。The plasma pharmacokinetics and tissue residues of doxycycline hydrochloride were investigated in pigs. The doxycycline hydroehloride concentrations in plasma and tissues were determined by using reversed-phase high performance liquid chromatography（RP-HPLC） after injecting a single intramuscular at dose of 2.5 mg/kg body weight （n= 6）. The kinetics of doxycycline hydrochloride was fittecl to one compartment model with first order absorption. The main parameters were as follows：the half-lives of absorption （t1/2 ka ） and elimination （t1/2 kc） were （0. 400±0. 312） h, and （9. 530 ±0. 956） h respectively, area under the plasma drug concentration-time curve from 0 to co（AUC） was （44. 414±4. 123） mg · h · L^-1 ,the peak plasma concentration （Cmax） was （2. 811±0. 136） mg/ L, peaking at （1. 910±0. 213） h （Tpn） after dosing. Moreover,each healthy pig was administered doxycycline hydrochloride i. m. once a day at dose of 2.5 mg/kg body weight for four days （n= 6）. The tissue residues of doxycycline hydrochloride were determined in muscle, liver, kidney, skin and fat. Tissue residues of doxycycline hydrochloride was to be lower to the maximum residue limit （MRL） after 16 days. The results indicated that the elimination of doxycycline hydrochloride in tissue was slow. It is proposed that the withdraw period should be not less than 16 days after an intramuscular injection at a dose of 2.5 mg/kg body weight.

关 键 词：猪 盐酸多西环素注射液 药物动力学 残留 

分 类 号：S859.7[农业科学—临床兽医学]