神经内分泌因子对体外培养新生犊牛肝细胞MTP mRNA表达的影响  

Effect of neuroendocrine factors on expression of MTP mRNA in newborn calf hepatocytes in vitro

作　　者：陈仕均[1,2] 唐海蓉[2] 刘国文[1] 王哲[1] 孙玉成[1] 邓俊良[3] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]河南科技学院,河南新乡453003 [3]四川农业大学动物科技学院,四川雅安625014

出　　处：《中国兽医学报》2008年第11期1321-1324,1328,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30230260,30571365,30600441)

摘　　要：为了阐明神经内分泌因子在奶牛肝脂蛋白组装与转运过程中的调控作用,通过向体外培养的新生犊牛肝细胞添加胰岛素、胰高血糖素和瘦蛋白,采用内对照RT-PCR方法检测神经内分泌因子对体外培养新生犊牛肝细胞微粒体甘油三酯转运蛋白(MTP)mRNA丰度的影响。结果显示,随着细胞培养液中胰岛素和胰高血糖素浓度升高,MTP mRNA的丰度呈现剂量依赖性下降(P<0.01);随着瘦蛋白浓度的增加,MTP mRNA的丰度先升高后降低(P<0.01或P<0.05)。这表明胰岛素和胰高血糖素对肝细胞MTP mRNA表达具有抑制作用,呈现剂量依赖性;瘦蛋白对肝细胞MTP mRNA表达具有低剂量促进而高剂量抑制的双重作用,且均呈现剂量依赖性。The present trail was conducted to light the regulating effect of neuroendocrine factors on assembly and secretion of lipoprotein in liver of dairy cows. Different concentrations of insulin, glucagons and leptin were added into monolayer culture media of primary-cultured newborn calf hepatocytes respectively. After 24 hours, abundance of MTP mRNA in primary-cultured newborn calf hepatocytes was detected by semiquantitative RT-PCR. The result showed that with increasing concentrations of insulin and glucagons, abundance of MTP mRNA in hepatocytes was down-regulated significantly with dose-dependence （P〈0.01）. However, with increasing concentrations of leptin,abundance of MTP mRNA in hepatocytes was up-regulated,and then decreased（P〈0.01 or P〈0.05）. It was concluded that neuroendocrine factors such as insulin,glucagons and leptin could regulated expression of MTP mRNA.

关 键 词：神经内分泌因子 犊牛肝细胞 MTP MRNA 体外培养 

分 类 号：S856.5[农业科学—临床兽医学] Q78[农业科学—兽医学]