EGCG氧化产物不同级分的分析及其抗氧化活性的研究  

Study on the Analysis and Antioxidant Activities of Different Extracts from (-)-Epigallocatechin Gallate Oxidation Products

作　　者：戚向阳[1,2] 

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100 [2]华中农业大学食品科技学院,湖北武汉430070

出　　处：《茶叶科学》2008年第6期436-442,共7页Journal of Tea Science

基　　金：国家十一五重点支撑项目(NO2006BAD27B00)

摘　　要：利用乙酸乙酯在中性和酸性条件下萃取EGCG氧化产物,得到级分A(EOPA)和级分B(EOPB),用化学发光法比较EGCG与EOPA、EOPB的抗氧化活性及其对DNA损伤的保护作用,并通过HPLC-MS分析两级分中的氧化产物。结果表明EGCG及EOPA、EOPB均有较好的清除活性氧和保护DNA损伤的活性,其中EOPA的活性强于未氧化的EGCG。HPLC-MS分析表明,EOPA、EOPB级分中的氧化产物均为二聚体。（-）-Epigallocatechin gallate （EGCG） was oxidized chemically and extracted under neutral, acidic condition value to obtain two extracts： fraction A （EOPA） and fraction B （EOPB）. The antioxidant activities of EOP A, EOP B and EGCG were determined using a chemiluminescence technique. The components of two fractions were analyzed by HPLC-ESI-MS. Results showed that the EOP A possessed the strongest antioxidant activity in all test system followed by EGCG, EOP B in a decreasing order. HPLC-ESI-MS analysis showed that the oxidation products of EOP A and EOPB were all dimmers.

关 键 词：EGCG氧化产物 化学发光 抗氧化 HPLC—ESI—MS 

分 类 号：R285[医药卫生—中药学]