天麻钩藤饮对胰岛素促自发性高血压乳鼠心肌成纤维细胞增殖的影响  

Effects of Tianma Gouteng Yin on Proliferation of Cardiac Fibroblasts of Spontaneous Hypertensive Rat Stimulated by Insulin

作　　者：黄习文[1] 孙敬和[1] 何玉萍[1] 冼绍祥[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405

出　　处：《中药新药与临床药理》2008年第6期430-434,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家中医药管理局课题(02-03JP34)。

摘　　要：目的观察天麻钩藤饮对胰岛素诱发的心肌成纤维细胞增殖升高的影响。方法从1日龄的自发性高血压乳鼠取得心肌,进行心肌成纤维细胞培养,并通过显微镜和免疫组化鉴定,观察天麻钩藤饮含药血清对胰岛素所致心肌成纤维细胞增殖升高的作用;以MTT比色法和3-H-TdR掺入法检测细胞增殖情况,以流式细胞仪检测细胞DNA周期。结果两种细胞增殖检测方法均显示天麻钩藤饮对胰岛素所致的心肌成纤维细胞增殖升高有抑制作用;胰岛素造模后细胞增殖升高主要是促进细胞从G0/G1期向S期转化,使细胞DNA合成增加而促进细胞增殖;天麻钩藤饮组与模型对照组比较G0/G1期百分率明显增加、S期百分率明显减少。结论天麻钩藤饮对胰岛素所致细胞增殖有抑制作用;可通过将细胞阻抑在G0/G1期,抑制其向S期转化从而抑制细胞增殖。这可能是天麻钩藤饮临床上治疗高血压病和防治心肌纤维化的作用机理之一。Objective To observe the effects of Tianma Gouteng Yin （TGY） on the proliferation of myocardial fibroblasts of spontaneous hypertensive rat（SHR） stimulated by insulin. Methods The cardiac fibroblasts from one- day-old spontaneously hypertensive rat were primarily cultured and then sub - cultured to 3rd - 6th generation, and were identified with light microscopy and immunohistochemical technique. The effect of serum containing TGY on the proliferation of cardiac fibroblasts of SHR treated with insulin was determined by MTT colorimetric assay and ^3- H - TdR incorporation, and the cell cycle was analysised with flow cytometer. Results TGY inhibited the proliferation of cardiac fibroblasts induced by insulin. Insulin can stimulate the proliferation of fibroblasts by promoting the cell cycle transfer from G0/G1 period to S period and increasing the synthesis of cell DNA. Compared with the model group, the percentage of the cells at G0/G1 period was much higher, while the percentage of the cells at S period was much lower in TGY group. Conclusion TGY has the effect on inhibiting the proliferation of cardiac fibroblasts and inhibiting the cell cycle transfer from G0/G1 period to S period, which may be one of its therapeutic and protective mechanisms for hypertension and myocardial fibrosis in clinic.

关 键 词：天麻钩藤饮 心肌成纤维细胞 胰岛素 自发性高血压大鼠 增殖 

分 类 号：R285.5[医药卫生—中药学]