不同野菊花提取物中蒙花苷和木犀草素的含量测定比较  

Content determination of linarin and luteolin from different extractions of Flos Chrysanthemi Indici

作　　者：毕跃峰[1] 符玲[1] 裴姗姗[1] 田新慧[1] 张小娟[1] 刘宏民[1] 

机构地区：[1]郑州大学新药研究开发中心,郑州450001

出　　处：《药物分析杂志》2008年第11期1797-1799,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技型中小企业技术创新项目(编号:07C26214100621)

摘　　要：目的:通过比较不同的提取方法对野菊花提取物中蒙花苷和木犀草素含量测定的影响,研究中药提取新工艺。方法:采用加热回流提取法、超声波提取法和组织破碎提取法3种提收工艺,同时提取野菊花中蒙花苷和木犀草素;利用RP-HPLC法,以水-乙腈-4%醋酸溶液为流动相(流速1.0 mL·min^(-1))司时测定蒙花苷和木犀草素含量。结果:蒙花苷和木犀草素分别在0.048～0.240 mg·mL^(-1)(r>0.9995)、0.0059～0.0294 mg·mL^(-1)(r>0.9999)浓度范围内线性关系良好,加热回流法、组织破碎法提取的蒙花苷和木犀草素含量均高于超声法,组织破碎法较回流法操作简便、节能节时,且可避免高温对热敏性物质的破坏性,表现出较高优越性。结论:组织破碎提取法有着高效经济的优势,是一种值得推广的高新提取工艺。Objective：To study the effects of different extraction methods on the content of linarin and luteolin in Flos Chrysanthemi Indiei. Method：Heating and refluxing extraction, ultrasonic extraction and smashing extraction were used respectively in the extraction of linarin and luteolin. The content of linarin and luteolin in Flos Chrysanthemi Indici were quantitated by using RP- HPLC on the YMC C18 （250 mm ×4.6 mm,5 μm ） colmnn with the mobile phase consisted of water - acetonitrile - 4% CH3 COOH （ flow rate ： 1.0 mL ·min^- 1 ） . Results： The linear ranges for linarin and luteolin were 0. 048 - 0. 240 mg·mL^- 1 （ r 〉 0. 9995 ） and 0. 0059 - 0. 0294 mg · mL^- 1 （ r〉 0. 9999） respectively. The content of linarin and luteolin extracted by smashing and refluxing extraction is much higher than that of ultrasonic extraction. Conclusion：Smashing extraction is obviously than the two other methods. And it is high -efficient, economical and may be worth while for popularization.

关 键 词：野菊花 野菊花提取物 蒙花苷 木犀草素 组织破碎提取法 

分 类 号：R917[医药卫生—药物分析学]