益母草药材与益母草注射液HPLC特征图谱相关性研究  

Studies on correlation for HPLC specific chromatogram of Leonurus japonicus Houtt and its injections

作　　者：姜卫东[1] 贺亚玲[1] 刘仲义[1] 

机构地区：[1]四川省食品药品检验所,成都610036

出　　处：《药物分析杂志》2008年第11期1854-1857,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技攻关项目"70种中药注射剂特征图谱研究及其技术平台建立"子课题(课题任务书编号:2001BA701A56);"益母草注射液指纹图研究"

摘　　要：目的:研究益母草药材与益母草注射液特征图谱的相关性。方法:应用反相HPLC色谱法,采用Aichrom Bond-AQ-C_(18)(5μm,4.6 mm×250 mm)色谱柱,以0.1 mol·L^(-1)磷酸二氢钠溶液(用0.1 mol·L^(-1)磷酸氢二钠溶液调pH至5.5)为流动相,流速1.0mL·min^(-1),检测波长242 nm。结果:对不同产地和采收期的益母草药材进行了特征图谱检测,以广元青川及西昌产盛花期药材与益母草注射液相关性最好。结论:本研究提供了益母草注射液生产用药材选择的方法。Objective ： Studies on correlation of HPLC specific chromatogram between Leonurus japonicus and its injections. Metbod：RP- HPLC condition included a Aichrom Bond- AQ- C18 （5 μm,4. 6 mm× 250 mm） column and the gradient elution was adopted with 0. 1 mol · L^-1 sodium dihydrogen phosphate （ adjusted pH value to 5.5 with 0. 1 mol ·L^-1disodium hydrogen phosphate）. The flow rate was 1.0 mL · min^-1, and the detection wavelength was 242 nm. Results： According to the Leonurus japonicus specific chromatogram of different producing areas and harvesting times, the correlation between the Leonurus japonicus injections and the full - bloom stage Leonurus japonicus of Qingchuan Guangyuan and Xichang is the best. Conclusion：This study is useful for manufacturers who can use HPLC specific chromatogram to select medicinal materials.

关 键 词：特征图谱 相关性 益母草药材 

分 类 号：R917[医药卫生—药物分析学]