抗生素微生物检定法-比浊法测定红霉素含量  

Microbial assay of erythromycin by turbidimetric method

作　　者：席志芳 衷红梅 党全训 绳芬 马泉荔 

机构地区：[1]陕西省药品检验所,西安710061

出　　处：《药物分析杂志》2008年第11期1865-1868,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立浊度法测定红霉素的效价。方法:以金黄色葡萄球菌[CMCC(B)26003]为实验菌,培养基为pH7.0的Ⅲ号培养基,缓冲液为pH7.8的磷酸盐缓冲液。结果:线性范围0.10～0.85 u·mL^(-1),r=0.9972;平均回收率为99.4%(n=9),与管碟法比较,结果无显著性差异。结论:本法快速、准确,可信限率优于管碟法,可作为红霉素含量测定方法。Objective：To establish a turbidimetric method for the microbial assay of erythromycin. Methods：The test microorganism was Staphylococcus aureus, pH7.0 medium m and pH7.8 phosphate buffer were employed. Results ： The calibration curve was linear in the concentration range of 0. 10 - 0. 85 u ·mL^-1, r = 0. 9972 ; The average recovery was 99.4% （ n = 9）. The results were accordant comparing with cylinder - plate method. Conclusion ： The methods is proved to be rapid,accurate and feasible to determine the content of erythromycin.

关 键 词：抗生素微生物检定 比浊法 红霉素 

分 类 号：R917[医药卫生—药物分析学]