几种中药中痕量啶虫眯和吡虫啉农药残留的胶束毛细管电泳在线推扫富集技术检测  

Determination of the trace pesticide residues of acetamiprid and imidacloprid in traditional Chinese medicines by micellar electrokinetic chromatography and on-line sweeping technique

作　　者：赵燕燕[1] 王翠玲[2] 苏芳[2] 王丽娟[2] 李月秋[1] 韩媛媛[1] 白洁[1] 

机构地区：[1]河北大学医学实验中心,保定071000 [2]河北大学药学院,保定071002

出　　处：《药物分析杂志》2008年第11期1873-1876,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：河北省科技攻关资助项目(05276101D-88;42761220);河北省中医药管理局资助项目(批准号:05015);河北大学人才引进项目(批准号:y2004039);河北大学自然科学基金项目(2005408)资助

摘　　要：目的:利用胶束毛细管电泳在线推扫富集技术建立测定几种中药中痕量啶虫眯和吡虫啉农药残留的方法。方法:中药样品通过甲醇超声提取,二氯甲烷萃取的方法处理。采用未涂层熔硅弹性石英毛细管(48.5μm×50 μm,有效柱长40 cm),选择20 mmol·L^(-1)硼砂和80 mmol·L^(-1)十二烷基硫酸钠(SDS)(H=6.93)为缓冲溶液,进样时间100 s,进样压力4.5kPa,分离电压15 kV,紫外检测波长262 mn,可实现啶虫眯和吡虫啉的分离检测。结果:在优化条件下,啶虫眯和吡虫啉的检出限分别为0.008,0.016 mg·kg^(-1);富集倍数分别为270和210倍。结论:本方法样品预处理操作简单、检测限低,能够用于中药中痕量啶虫眯和吡虫啉农药残留的检测。Objective：A micellar electrokinetic chromatography（MEKC） method based on on -line sweeping technique was developed for determination of trace acetamiprid and imidacloprid in traditional Chinese medicines. Methods：The traditional Chinese medicines samples, to which some methanol was first added, were extracted by ultrasonic, and leached by dichloromethane and in the running buffer of 20 mmol · L^-1Na2B4O7 ＋ 80 mmol·L^-1 sodium dodecyl sulfate （SDS） , pH = 6.93, the acetamiprid and imidacloprid in samples were successfully＇ separated and detected within 100 s, in a non - coating fused - silica capillary column（48.5 cm × 50μm i. d. , effective length 40 cm）at separation voltage of 15 kV,and the injection pressure of 4. 5 kPa,with UV detection at 262 nm. Results：The detection limits of acetamiprid and imidacloprid were 0. 008 mg · kg^-1 and 0. 016 mg · kg^-1, respectively. This method allows of up to 270 - and 210 - fold accumulation concentration of acetamiprid and imidacloprid. Conclusions：This method can make the pretreatment of the traditional Chinese medicine samples simple and the detection limits are low. It can be used to separate and determine the trace pesticide residuals of acetamiprid and imidacloprid in traditional Chinese medicines.

关 键 词：胶束毛细管电泳 在线推扫富集技术 痕量 啶虫眯 吡虫啉 中药 

分 类 号：R917[医药卫生—药物分析学]