高糖对牙周韧带细胞中转录因子Scleraxis表达的影响  

Effect of high glucose on the expression of transcription factor Scleraxis in periodontal ligament cells in vitro

作　　者：袁亚娣[1] 苗松[2] 谢昊[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,430079 [2]广州市花都区胡忠医院口腔科

出　　处：《中华口腔医学杂志》2008年第11期668-670,共3页Chinese Journal of Stomatology

摘　　要：目的通过研究高糖对牙周韧带细胞（priodontal ligament cells，PDLC）中转录因子Scleraxis表达的影响，探讨高糖抑制PDLC分化及糖尿病患者牙周病愈合延迟的分子机制。方法分别运用高糖型和低糖型DMEM培养基体外分离培养人PDLC，然后检测相应培养条件下碱性磷酸酶（ALP）活性和Scleraxis mRNA的表达。其中，ALP活性采用生物化学方法定量检测，Scleraxis mRNA的表达通过反转录聚合酶链反应（RT-PCR）技术半定量检测。结果与低糖型DMEM培养基培养的PDLC相比，高糖培养条件下PDLC的ALP活性下降，分别为0．218±0．012和0．113±0．068；而Scleraxis的表达则增加，分别为0．611±0．205和0．973±0．055。经两独立样本t检验，ALP活性和Scleraxis表达的变化差异均有统计学意义（P〈0．05）。结论糖尿病患者的高血糖水平可能通过上调Scleraxis的表达来抑制PDLC向成骨细胞分化，进而影响牙周病的愈合。Objective To approach the mechanisms of effects of high glucose on the differentiation of periodontal ligament cells （PDLC） by investigating the changes of Scleraxis mRNA expression in high glucose condition in vitro. Methods Human PDLC were cultured in high glucose medium （4500 mg/L glucose） and normal glucose medium（ 1000 mg/L glucose） ,respectively. High glucose was uesd to inhibit the osteogenic differentiation of PDLC. PDLC cultured in normal glucose medium served as control. Alkaline phosphatase（ALP） activity, the early parameter of osteogenetic differentiation of cells and the expression of Scleraxis mRNA were detected in each group. ALP activity was measured colourmetrically by using nitrophenyl phosphate as a substrate and the expression of Scleraxis mRNA was analyzed by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results ALP activity of PDLC was lower in high glucose medium than in normal glucose medium, and the values were 0. 113 ± 0. 068 and 0. 218 ± 0. 012, respectively. However, the level of Scleraxis mRNA was quite higher in high glucose midium compared with in normal glucose medium, and the values were 0. 973 ± 0. 055 and 0. 611 ± 0. 205, respectively. The values of ALP activity and the expression of Scleraxis mRNA were significantly different between the two groups. Conclusions High glucose inhibited osteogenetic differentiation of PDLC and upregulated Scleraxis expression. The adverse changes of Scleraxis expression and osteogenic differentiation of PDLC suggest that Scleraxis may regulate the osteogenic differentiation of PDLC negatively and the inhibition of high glucose on osteogenetic differentiation of PDLC may be regulated by Scleraxis in transcription level.

关 键 词：牙周膜 转录因子 葡萄糖 SCLERAXIS 

分 类 号：R686[医药卫生—骨科学]