水稻香味基因的精细定位  

Fine Mapping of the Fragrance Gene in Rice

作　　者：张涛[1] 张红宇[2] 蒋开锋[1] 徐培州[2] 汪旭东[2] 吴先军[2] 郑家奎[1] 

机构地区：[1]四川农业科学院水稻高粱研究所,德阳618000 [2]四川农业大学水稻研究所,温江611130

出　　处：《分子植物育种》2008年第6期1038-1044,共7页Molecular Plant Breeding

基　　金：农业部跨越计划--长江上游一季超级稻品种选育研究项目、四川省青年基金项目(08ZQ026-043);四川省农科院青年基金项目;四川十一五水稻育种攻关项目等基金项目资助

摘　　要：随着现代生物技术的发展,越来越多的报道证明,香稻的香味物质为2-乙酰-1-吡咯啉,水稻的香味性状受位于水稻第8染色体上的1对隐性基因控制。本研究以11个香稻品种和4个非香稻品种为材料,分析了香味基因的遗传;以茉莉花香型的粳稻品种粳香米和非香品种MR63的F2群体为材料,对控制香味性状的基因进行了精细定位。结果表明,水稻香味受1对隐性基因控制,并将香味基因定位在水稻第8染色体上,位于InDeL标记AP05537-17和AP004463-13之间,与标记AP004463-13间的距离为0.4cM,与标记AP05537-17间的距离为1.6cM,在物理图谱上的物理距离大约252kb。分析发现BAC克隆AP004463最有可能包含该基因。最终在该区域内预测有15个基因可能与该香味基因相关。With the development of modem biotechnology, the flavour or fragrance of aromatic rice is associated with the presence of 2-acetyl-1-pyrroline. A recessive gene （fgr） on chromosome 8 office has been linked to this important trait. In the present study, a F2 segregation population derived from a cross between Jingxiangmi with jasmine fragrant and MR63 without fragrance were used to study the inheritance of fragrance and map the flagrance gene in rice chromosome 8. Genetic mapping of molecular markers and the fragrance phenotype demonstrated that the InDel markers AP005537-17 and AP004463-13 flankedfgr, The physical distance between AP005537-17 and AP004463-13 is about 252 kb. The data suggested thatfgr was closer to AP004463-13 （0.4 cM） than to AP005537-17 （1.6 cM）. Data analysis suggested that one Bacterial Artificial Chromosomes （BAC） （clone AP004463） was most likely to contain the gene. Ultimate forecast in the region may have 15 genes with the genes related to fragrance.

关 键 词：香稻 SSR标记 香味基因 克隆 

分 类 号：S511[农业科学—作物学] TP212[自动化与计算机技术—检测技术与自动化装置]