Albumin resuscitation protects against traumatic/hemorrhagic shock-induced lung apoptosis in rats  

作　　者：Yun ZHANG Zhong-yan LIANG Shao-yang ZHANG Fang-fang HUANG Wei WU Yuan GAO Zuo-bing CHEN 

机构地区：[1]Department of Emergency Surgery, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China [2]Department of Intensive Care Unit, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China [3]Institute of lnfectious Diseases, the First Affiliated Hospital School of Medicine, Zhejiang University, Hangzhou 310003, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2008年第11期871-878,共8页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China (No. 30672071) ;the Traditional Chinese Medicine Foun- dation of Zhejiang Province, China (No. 2004C071)

摘　　要：Objective: To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats. Methods: Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer's lactate solution (RS) or 5% (w/v) albumin (ALB), and the left lower lobes of the lungs were resected. Results: Albumin administered during reperfusion markedly attenuated injury of the lung and decreased the concentration of lactic acid and the number of in situ TdT-mediated dUTP nick-end labelling (TUNEL)-positive cells. Moreover, immunohistochemistry performed 24 h after reperfusion revealed increases in the level of nuclear factor κB (NF-κB), and phosphorylated p38 mitogen-activated protein kinase (MAPK) in the albumin-untreated group was down-regulated by albumin treatment when compared with the sham rats. Conclusion: Resuscitation with albumin attenuates tissue injury and inhibits T/HS-induced apoptosis in the lung via the p38 MAPK signal transduction pathway that functions to stimulate the activation of NF-κB.Objective： To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock （T/HS） rats. Methods： Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer＇s lactate solution （RS） or 5% （w/v） albumin （ALB）, and the left lower lobes of the lungs were resected. Results： Albumin administered during reperfusion markedly attenuated injury of the lung and decreased the concentration of lactic acid and the number of in situ TdT-mediated dUTP nick-end labelling （TUNEL）-positive cells. Moreover, immunohistochemistry performed 24 h after reperfusion revealed increases in the level of nuclear factor κB （NF-κB）, and phosphorylated p38 mitogen-activated protein kinase （MAPK） in the albumin-untreated group was down-regulated by albumin treatment when compared with the sham rats. Conclusion： Resuscitation with albumin attenuates tissue injury and inhibits T/HS-induced apoptosis in the lung via the p38 MAPK signal transduction pathway that functions to stimulate the activation of NF-κB.

关 键 词：Traumatic/hemorrhagic shock （T/HS） Mitogen-activated protein kinase （MAPK） Nuclear factor κB （NF-κB） ALBUMIN APOPTOSIS 

分 类 号：R64[医药卫生—外科学]