人参皂甙Rg1对原代培养胎鼠脑神经细胞存活和可塑性的影响  

Effect of Ginsenoside Rg1 on the Survival Rate and Neuronal Plasticity of Primary Cultured Cortical Neurons

作　　者：彭涛[1] 刘英辉[2] 陈绍宏[1] 张晓云[1] 

机构地区：[1]成都中医药大学附属医院,四川成都610075 [2]四川大学基础医学与法医学院,四川成都610041

出　　处：《华西医学》2008年第1期106-107,共2页West China Medical Journal

摘　　要：目的:研究人参皂甙Rg1对原代培养胎鼠脑神经细胞存活和可塑性的影响。方法:实验分为:实验组(人参皂甙Rg11mg/L,10mg/L,100mg/L),阳性药物对照组(bFGF20μg/L)以及空白对照组。相差倒置显微镜观察细胞生长情况,并测量细胞突起的长度;用MTT法测定培养细胞的存活率;Western-blot法检测神经生长相关蛋白GAP-43和神经丝蛋白NF-200的表达。结果:(1)细胞平均突起长度:实验高中剂量组神经元突起的平均长度均长于对照组。(2)MTT值:实验高中低剂量组的灰度均明显大于对照组。(3)GAP43和NF200的表达:实验高中剂量组的蛋白表达均明显大于对照组。结论:人参皂甙Rg1对于体外培养的胎鼠脑神经细胞的存活有较强的维持作用,并能促进突起生长,使神经可塑性相关蛋白表达上调。Objective：To study the effect of Ginsenoside Rg1 on the survival rate and neuronal plasticity of primary cultured cortical neurons.Methods：Cortical neurons obtained from fetal rats were cultured in culture media supplemented with Ginsenoside Rg1（1mg/L,10mg/L,100mg/L）and bFGF（0μg/L）respectively.Neurons cultured in non-supplemented media served as contro1.Phase-contrast microscope observations,measurement of neurite length,and MTT methods were used to evaluate the survival and activity of the cultured neurons.Western-blot was applied to detect the expression level of Growth Associated Protein-43（GAP43） and Neurofilament-200（NF200）.Result：（1）Neurite length of neurons in the experimental groups（10mg/L,100mg/L）was longer than in the control.（2）Cell survival rate in all the experimental groups was superior to that in the control group in MTT values.（3）The expression level of GAP-43 and NF-200 of the experimental groups（10mg/L,100mg/L）was longer than those of the control.Conclusion：Ginsenoside Rg1 can enhance neuronal survival,promote axonal growth,and increase the expression of neuronal plasticity associated proteins in vitro.

关 键 词：人参皂甙RG1 神经元 神经可塑性 GAP43 NF200 

分 类 号：R285[医药卫生—中药学]