辣椒碱对小菜蛾的驱避活性及其体内谷胱甘肽-S-转移酶和Na^+,K^+-ATP酶活性的影响  

Repellent activity of capsaicin and its effects on glutathione-S-transferase and Na^+,K^+-ATPase activity in Plutella xylostella(Lepidoptera:Plutellidae)

作　　者：纪明山[1] 刘少武[1] 谷祖敏[1] 魏松红[1] 王英姿[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁省生物农药工程技术研究中心,沈阳110161

出　　处：《昆虫学报》2008年第10期1039-1043,共5页Acta Entomologica Sinica

基　　金：辽宁省"十一五"科技攻关项目(2006215004);辽宁省工程技术研究中心专项(200535)

摘　　要：采用常规生测方法和酶活力测定方法,初步研究了辣椒碱对小菜蛾Plutella xylostella L.的产卵忌避和拒食作用,及其对小菜蛾体内谷胱甘肽-S-转移酶、Na+,K+-ATP酶活性的影响,以期阐明辣椒碱对害虫的作用机制。结果表明,辣椒碱对小菜蛾表现出较强的产卵忌避活性和拒食活性。在6.25×104mg/L浓度下,处理24h辣椒碱对小菜蛾的非选择性产卵忌避率达96.55%,选择性产卵忌避率为84.30%;在相同浓度下,处理48h辣椒碱对小菜蛾的非选择性拒食率达81.47%,选择性拒食率为69.69%。另外,经1.25×105mg/L辣椒碱不同时间处理后,小菜蛾体内的谷胱甘肽-S-转移酶酶活力和Na+,K+-ATP酶活力与对照相比均产生了波动,处理18h时小菜蛾体内GSTs活力最高,为152.01U·mg-1pro·min-1,处理1h时小菜蛾体内Na+,K+-ATP酶活力最高,为19.99U·mg-1pro·min-1。结果说明辣椒碱能够影响小菜蛾产卵和取食行为,并且对其体内的酶系也产生了影响。The repellent activity of capsaicin against PluteUa xylostella, and the effects of capsaicin on activities of glutathione-S-transferase and Na^＋ , K^＋ -ATPase in the 3rd instar larvae of the moth were tested in the laboratory with conventional methods. The results showed that capsaicin had strong oviposition deterrence and antifeedant activity against P. xylostella. At a concentration of 6.25 × 10^4 mg/L, the nonselective oviposition deterrence rate was 96.55% 24 h after capsaicin application, while the selective oviposition deterrence rate was 84.30%. At the same concentration, the nonselective antifeedant rate and selective antifeedant rate was 81.47% and 69.69% respectively 48 h after capsaicin application. In addition, glutathione-S-transferase and Na^＋, K^＋ -ATPase activities fluctuated after P. xylostella was treated with capsaicin for different hours. When P. xylostella was treated with 1.25× 10^5 mg/L capsaicin, the highest GSTs and ATPase activities were found at 18 h and 1 h after treatment, respectively. These results suggested that capsaicin can disturb the oviposition and feeding of the moth, and influence some enzymes in its body.

关 键 词：小菜蛾 辣椒碱 产卵忌避作用 拒食作用 谷胱甘肽-S-转移酶 NA^+ K^+-ATP酶 

分 类 号：Q966[生物学—昆虫学]