基于16S rDNA和28S rDNA D2基因序列与形态特征联合分析的中国角顶叶蝉亚科系统发育研究(半翅目:叶蝉科)(英文)  

Phylogeny of Deltocephalinae(Hemiptera:Cicadellidae) from China based on partial 16S rDNA and 28S rDNA D2 sequences combined with morphological characters

作　　者：戴仁怀[1,2] 陈学新[2] 李子忠[1] 

机构地区：[1]贵州大学昆虫研究所,贵州山地农业病虫害重点实验室,贵阳550025 [2]浙江大学植物保护系,杭州310029

出　　处：《昆虫学报》2008年第10期1055-1064,共10页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30270176);贵州省优秀科技教育人才省长专项基金(黔省专合字(2006)9号);贵州大学人才科研基金资助项目

摘　　要：首次在国内利用28S rDNA D2区段和16S rDNA基因序列,结合50个形态特征对角顶叶蝉亚科(Deltocephalinae)[半翅目(Hemiptera):叶蝉科(Cicadellidae)]19个属进行系统发育分析研究。从无水乙醇浸泡保存的标本中提取基因组DNA并扩增了19个内群和1种外群Typhlocybinae[半翅目(Hemiptera):叶蝉科(Cicadellidae)]种类的28S rDNA D2基因片段并测序,同时扩增了16SrDNA基因片段并测序11条,采用了GenBank中1个种类的16S rDNA同源序列。采用PAUP*4.0和MrBayes3.0两个分析软件和3种建树方法,利用同源28S D2 rDNA和16S rDNA两个基因序列与形态特征结合进行系统发育分析研究。分析结果表明,二叉叶蝉族Macrostelini是一个单系,并在角顶叶蝉亚科的系统发育中处于基部的位置,是内群中最原始的族;角顶叶蝉族Deltocephalini中除了纹翅叶蝉属Nakaharanus,其余各属构成单系;殃叶蝉族Euscelini内属的归属比较混乱,可能是一个并系群,属间差异有待进一步研究。隆额叶蝉族Paralimnini与顶带叶蝉族Athysanini是姐妹群。带叶蝉属Scaphoideus与纹翅叶蝉属Nakaharanus是姐妹群,二者与木叶蝉属Phlogotettix的关系最近,三者构成一个单系,建议将三者归为带叶蝉族Scaphoideini。研究结果还表明,小眼叶蝉族Xestocephalini和Balcluthini的系统发育位置不明,有待进一步研究。The phylogeny of 19 genera of Dehocephalinae leafhoppers was analyzed based on 50 adult morphological characters combined with nucleotide sequences of the mitochondrial 16S rDNA and nuclear 28S D2 rDNA genes. One species of Typhlocybinae was included as outgroup. Parsimonian, distance and Bayesian methods were used to estimate the phylogenetic relationships. The topology of the phylogenetic trees generated with different methods was quite similar. We partially resolved the morphologically-defined tribes and the relationships among 19 genera of Dehocephalinae. The genus Macrosteles was well supported to occupy a basal position in the study, so the most primary tribe in Dehocephalinae might be Macrostelini. The phylogenetic analysis trees put all genera of Deltocephalini but Nakaharanus onto a single lineage. The genus Balclutha, corresponding to the tribe Balcluthini, remains unsolved in our analyses. The Euscelini might be a polyphyletic group in the analysis. Analytical result recovered Athysanini and Paralimnini as monophyletic clades. The clade Phlogotettix and Scaphoideus-Nakaharanus was constantly resolved using different methods. We suggested that Scaphoideus, Nakaharanus and Phlogotettix should be included in or into Scaphoideini. But the results resolved poorly the taxonomic status of Xestocephalini overall.

关 键 词：半翅目 叶蝉科 角顶叶蝉亚科 16SrDNA 28S D2rDNA 形态特征 系统发育 

分 类 号：Q969.35[生物学—昆虫学]