不同代次S191株麻疹病毒的基因序列分析  

Gene Sequencing of Measles Virus S191 Strain of Various Passages

作　　者：魏树源[1] 祁春华[1] 陈晓琦[1] 桂金柱[1] 王珍[1] 

机构地区：[1]武汉生物制品研究所麻腮疫苗室,武汉430060

出　　处：《中国生物制品学杂志》2008年第11期941-944,共4页Chinese Journal of Biologicals

摘　　要：目的分析不同代次S191纯化株麻疹病毒(MV)的主要蛋白基因序列。方法将S191纯化株MV在原代鸡胚细胞(CEC)上连续传4代,观察每代病毒培养特性。选择其中的CEC28、30和31代病毒,测定主要蛋白N、M、F和H基因序列,并与GenBank中标准株S191(1994)和S191(2008)序列进行比对。结果S191纯化株病毒在CEC上连续传4代,其培养特性、致细胞病变效应(CPE)及病毒滴度均基本稳定。S191株病毒CEC各代与S191(1994)株比较,在15个核苷酸位点上有区别,其中N蛋白基因6个、M蛋白基因3个、H蛋白基因1个核苷酸的差异对其编码的氨基酸产生了影响;与S191(2008)株比较,在2个核苷酸位点上有区别,且均导致氨基酸的改变。各代之间主要蛋白基因未见突变。结论S191纯化株病毒在《中国药典》三部(2005版)规定的代次范围内比较稳定,在CEC30代内均可作为工作代毒种使用。Objective To analyze the gene sequence of major protein of purified measles virus （MV） S191 strain of various passages. Methods Subculture purified MV strain S191 in primary chick embryo cells （CECs） for 4 passages and observe the cultural characters of each passage. The genes encoding major protein of MV strain S191 of passages 28, 30 and 31 were sequenced, and the results were compared with those of standard strains S191 （1994） and S191 （2008） reported in GenBank. Results After subculture in CECs for 4 passages, the cultural characters, CPE and titer of S191 strain showed no significant change. The S191 strain of various passages showed difference with standard strain S191 （1994） in 15 nucleotide sites, of which N protein gene in 6, M protein gene in 3 and H protein gene in 1 site, resulting the change of the corresponding amino acids. Compared with S191 （2008） strain, S191 strain showed difference in 2 nucleotide sites which also resulted in the change of amino acids. No mutation was observed in major protein gene of S191 strain of various passages. Conclusion The purified S191 strain is stable within the passages specified in Chinese Pharmacopoeia （2005 edition）, and that within 30 passages in CEC may be used as working virus seeds.

关 键 词：麻疹病毒 S191株 蛋白基因 序列分析 

分 类 号：R373.11[医药卫生—病原生物学]