尾加压素Ⅱ对体外高糖培养大鼠肾小球系膜细胞周期的影响  

Effect of Urotensin Ⅱ on Cycle of Rat Mesangial Cells Cultured in High Glucose Medium In Vitro

作　　者：赵岩[1,2] 李才[1] 林风武[3] 田琳[1] 

机构地区：[1]吉林大学药学院实验药理与毒理学教研室,长春130021 [2]吉林大学第二医院内分泌科,长春130041 [3]吉林大学中日联谊医院胸外科,长春130061

出　　处：《中国生物制品学杂志》2008年第11期954-956,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助课题(30570855)

摘　　要：目的观察尾加压素Ⅱ(UⅡ)对体外高糖培养的大鼠肾小球系膜细胞周期的影响。方法体外高糖培养大鼠肾小球系膜细胞,加入不同浓度的UⅡ(10-7、10-8、10-9和10-10mol/L),37℃孵育24h,用碘化丙啶染色流式细胞术分析肾小球系膜细胞周期分布。结果UⅡ对体外高糖培养的大鼠肾小球系膜细胞有明显的促增殖作用,表现为细胞周期S期比例增加,以浓度为10-8mol/L时最明显。Ca2+阻断剂尼卡地平、Ca2+螯合剂EDTA及抗UⅡ抗体能明显抑制其促增殖作用。结论UⅡ能提高体外高糖培养的大鼠肾小球系膜细胞S期DNA合成速率,提示UⅡ对其具有明显的促增殖作用。Objective To observe the effect of urotensin Ⅱ （U Ⅱ ） on the cycle of rat mesangial ceils cultured in high glucose medium in vitro. Methods Add the U Ⅱ at concentrations of 10^-7, 10^-8, 10^-9 and 10^-10 mol/L to the rat mesangial cells cultured in high glucose medium in vitro respectively. Incubate the mixtures at 37℃ for 24 h and determine the cycle of rat mesangial cells by flow cytometry with PI staining. Results U Ⅱ ,especially at a concentration of 104 mol / L, promoted the proliferation of rat mesangial cells cultured in high glucose medium in vitro significantly, expressed as the increased percentage of cells at S stage. The promoting effect was significantly inhibited by calcium ion blocker nicardipiue, calcium ion chalating agent EDTA and antibody against U Ⅱ . Conclusion U Ⅱ inereased the rate of DNA synthesis at S stage of rat mesangial cells cultured in high glucose medium in vitro, indicating the significantly promoting effect of U Ⅱ on proliferation of the cells.

关 键 词：尾加压素Ⅱ 系膜细胞 细胞周期 糖尿病肾病 

分 类 号：R692.6[医药卫生—泌尿科学]