塞来昔布促进顺铂诱导人骨肉瘤细胞MG-63凋亡的作用  

Enhancement of Cisplatin-induced Apoptosis of Human Osteosarcoma MG-63 Cells by Celecoxib

作　　者：史晓红[1] 黄朝梁[1] 

机构地区：[1]重庆医科大学第二附属医院骨科,重庆400010

出　　处：《中国生物制品学杂志》2008年第11期992-994,共3页Chinese Journal of Biologicals

摘　　要：目的探讨塞来昔布促进顺铂诱导人骨肉瘤细胞MG-63凋亡的作用。方法将人骨肉瘤细胞分为塞来昔布组、顺铂组和联合组,分别加入不同浓度的塞来昔布、顺铂和塞来昔布+顺铂,采用MTT法检测细胞活性;流式细胞术检测细胞凋亡比例;吖啶橙/溴化乙锭(AO/EB)双染色观察细胞并计算凋亡率。结果塞来昔布单独作用于MG-63细胞后,细胞抑制率随药物浓度的升高而升高;联合组细胞抑制率均较顺铂组明显升高;FCM分析联合组细胞凋亡比例明显上升;AO/EB染色结果显示,联合组的细胞凋亡率明显高于塞来昔布组和顺铂组。结论塞来昔布和顺铂均可引起人骨肉瘤细胞的凋亡,两者联合使用可明显增强对人骨肉瘤细胞的凋亡作用。O bjective To explore the enhancement of cisplatin-induced apoptosis of human osteosarcoma MG-63 cells by celecoxib. Methods Divide MG-63 cells into 3 groups and treat with celecoxib, cisplatin and celecoxib ＋ cisplatin respectively. Determine the cell activity by MTT method and the proportion of apoptotie cells by flow eytometry. Observe the apoptosis of cells by AO / EB staining and calculate the apoptosis rate. Results After treatment with celecoxib alone, the inhibiting rate of MG-63 cells increased with the increasing concentration of drug. However, the inhibiting rate of cells treated with eeleeoxib ＋ cisplatin was significantly higher than that with cisplatin alone. FCM analysis proved that the proportion of apoptotie cells treated with eelecoxib ＋ cisplatin increased significantly. AO / EB staining showed that the apoptosis rate of cells treated with eelecoxib ＋ cisplatin was significantly higher than that with celecoxib or cisplatin alone. Conclusion Both celecoxib and eisplatin induced the apoptosis of human osteosarcoma MG-63 cells. However, the combination of the two drugs enhanced the apoptosis significantly.

关 键 词：骨肉瘤 塞来昔布 顺铂 细胞凋亡 

分 类 号：R979.12[医药卫生—药品] Q255[医药卫生—药学]