自身免疫病患者血液中穿通支原体分离培养及其血清IL-6与TNF-α的检测  

Isolation of Mycoplasma penetrans and detection of the levels of serum IL-6 and TNF-α in the blood of patients with antoimmune disease

作　　者：温秀姝[1] 王澄[1] 周丽萍[1] 王赛芳[1] 刘欢乐[1] 

机构地区：[1]温州医学院附属第一医院检验科,浙江温州325000

出　　处：《细胞与分子免疫学杂志》2008年第11期1079-1081,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：浙江省自然科学基金资助项目(399066)

摘　　要：目的:探讨自身免疫病AID患者血液中穿通支原体(Mpe)的分离检出以及Mpe感染患者血清中IL-6与TNF-α的浓度水平。方法:采用分离培养共计从44例AID患者血液标本,16例ASO或RF阳性体检人员及28例正常对照相应标本中进行支原体分离检测,对培养阳性菌株采用穿通及发酵支原体套式PCR予以证实,并用RIA对相应血清进行IL-6及TNF-α浓度检测。结果:于17例(38.6%)AID患者血液中分离到Mpe,2例(12.5%)ASO或RF阳性体检人员中分离到Mpe,而正常对照组中则无1例阳性,组间支原体检出率有统计学意义(P<0.01)。支原体阳性AID组血液IL-6浓度(3.30±1.49)μg/L与支原体阴性AID组(2.43±0.95)μg/L及正常对照组(1.14±0.32)μg/L之间差异有统计学意义(P<0.01);支原体阳性AID组血液TNF-α浓度(293.3±179.9)ng/L与支原体阴性AID组(173.9±73.9)ng/L及正常对照组(108.8±33.8)ng/L组之间也有统计学意义(P<0.01)。结论:Mpe感染与AID的发生密切相关,Mpe感染患者血清中IL-6与TNF-α较非感染对照人群明显升高。AIM： To investigate the isolation of Mycoplasma penetrans from the blood of autoimmune disease patients and to evaluate the levels of serum interleukin-6 （IL-6） and tumor necrosis factor-alpha （TNF-α） in patients with autoimmune disease （AID）. METHODS： M.penetrans was isolated and cultured from the blood specimens of 44 patients with AID. Among them 16 patients were control groupⅠ, who were the objects. 28 patients were in control groupⅡ as contrast. The serum ASO or RF level of the patients in control groupⅠ was higher than that in control group Ⅱ. The positive specimens were confirmed by nPCR and the levels of IL-6 and TNF-α were measured by RIA in the blood samples. RESULTS： M.penetrans was detected in the blood of 17 patients and the positive detection rate was 38.6% （17/44） in AID group. There was significant difference between the detection rate from group Ⅰ （12.5%, 2/16, P〈0.01） and that in groupⅡ （0%, 0/28, P〈0.01）. The serum level of IL-6 in the AID with M.penetrans infection patients in group Ⅰ （3.30±1.49） μg/L was significantly different from that in the AID without M.penetrans infection patients in groupⅠ （2.43±0.95） μg/L and that in group Ⅱ（1.14±0.32） μg/L, P〈0.01. The serum level of TNF-α in the AID with M.penetrans infection patients in groupⅠ （293.3±179.9） ng/L was significantly different from that in the AID without M.penetrans infection patients in groupⅠ（173.9±73.9） ng/L and that in groupⅡ（108.8±33.8） ng/L, P〈0.01.CONCLUSION： M.penetrans occurs with high frequency in the blood of autoimmune disease patients. The evident increase of serum levels of IL-6 and TNF-α in AID with M.penetrans infection than the control groups.

关 键 词：穿通支原体 自身免疫病 血液 IL-6 TNF-Α 

分 类 号：R392.11[医药卫生—免疫学]