原花青素对2型糖尿病局灶性脑缺血大鼠海马组织兴奋性氨基酸的影响  

Effect of procyanidin on excitatory amino acids of hippocampus in type 2 diabetes mellitus rats with focal cerebral ischemia

作　　者：韩苗苗[1] 闵连秋[1] 宋晓伟[1] 

机构地区：[1]辽宁医学院附属第一医院神经内科,辽宁锦州121001

出　　处：《中国新药与临床杂志》2008年第10期772-774,共3页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的探讨原花青素(PC)对2型糖尿病局灶性脑缺血大鼠海马组织兴奋性氨基酸(EAA)的影响。方法通过高脂高糖饮食4 wk,尾静脉注射小剂量链脲佐菌素(STZ)25.0 mg·kg^(-1)制备2型糖尿病大鼠模型,2 wk后建立局灶性脑缺血模型,24 h后处死,高效液相色谱法测模型大鼠海马组织EAA,观察PC(50、100、200 mg·kg^(-1))对EAA的影响。结果与假手术组比较,普通脑缺血大鼠模型和2型糖尿病缺血模型谷氨酸(Glu)和天冬氨酸(ASP)及抑制性氨基酸γ-氨基丁酸(CABA)均有升高(P<0.05,P<0.01),但2组间无明显差异(P>0.05),对甘氨酸(Gly)无明显影响(P>0.05);与2型糖尿病缺血模型组相比,PC中、高剂量组Glu、ASP显著下降(P<0.01),各剂量组对GABA和Gly无明显影响。结论PC可能通过对抗EAA毒性实现对2型糖尿病局灶性脑缺血大鼠的保护作用。AIM To investigate the effect of procyanidin （PC） on the excitatory amino acids （EAA） of hippocampus in type 2 diabetes mellitus （T2DM） rats with focal cerebral ischemia. METHODS T2DM rats were made by feeding fat and glucose forage for 4 wk, and injecting streptozotocin （STZ） through caudal vein in low dose （25.0 mg·kg^-1） . Two weeks later, the focal cerebral isehemia modes were made and rats were killed 24 h later, and the effect of PC （50, 100, 200 mg·kg^-1） on EAA in hippocampus was observed by high performance liquid chromatograph. RESULTS Compared with sham-operation group, the glutamic acid （Glu）, aminosuccinic acid （ASP） and gamma-amionbutyric acid （GABA） increased in common and T2DM model rats （P 〈 0.05, P 〈 0.01 ） with no difference for the two groups （P 〉 0.05） , and there were no difference in glycine （Gly） （P 〉 0.05） in the two groups. Compared with T2DM model group, Glu and ASP of mid-dose and high-dose groups decreased （P 〈 0.01, P 〈 0.05）, and PC had no effect on GABA and Gly in all dose groups （P 〉 0.05）. CONCLUSION By resisting to the toxicity of EAA, PC may realize the neuroprotective effect on ischemic rats of type 2 diabetes mellitus.

关 键 词：花青苷类 糖尿病 2型 兴奋性氨基酸类 脑缺血 

分 类 号：R971.6[医药卫生—药品]