Cyr61、TNF-α、IL-6和IL-1β与颈动脉损伤后再狭窄的关系  

Relationship between expression of Cyr61,TNF-α,IL-6,IL-1βand restenosis of injured carotid artery

作　　者：张春炳[1] 朱健[1] 张秋玉[2] 吴娟娟[2] 沈佰华[2] 

机构地区：[1]上海交通大学医学院第九人民医院急诊科,上海200011 [2]上海交通大学医学院上海市免疫学研究所,上海200025

出　　处：《上海交通大学学报（医学版）》2008年第11期1410-1415,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海交通大学医学院科技基金项目(05XJ21027)~~

摘　　要：目的探讨富含半胱氨酸61蛋白(Cyr61)及组织炎症因子TNF-α、IL-6、IL-1β与颈动脉损伤后再狭窄发生的关系。方法36只Wistar大鼠随机分为模型组和对照组(n=18)。模型组大鼠经2F Fogarty球囊导管损伤左颈总动脉建立颈动脉血管成形术模型,分别于建模后1、4、7、14、28 d和3月取材,观察血管壁组织的病理学改变;免疫组化和Rea1-time PCR技术检测血管壁组织中Cyr61、TNF-α、IL-6和IL-1β的表达变化;ELISA法测定血清TNF-α、IL-6和IL-1β水平。结果球囊损伤后血管壁新生内膜在术后7 d出现,术后28 d时达到高峰;形成的新生内膜厚度不均,较对照组明显增厚(P<0.01);大鼠颈总动脉损伤后,血管壁组织Cyr61、TNF-α、IL-6和IL-1β表达明显升高,与对照组相比差异有统计学意义(P<0.05);而血清炎症因子水平无明显变化。结论大鼠颈总动脉球囊损伤后血管壁Cyr61、TNF-α、IL-6和IL-1β表达明显增强,提示Cyr61可能与炎症因子相互协同作用,使血管壁组织增生而最终导致再狭窄的发生。Objective To investigate the relationship between the expression of cysteine-rich 61 （Cyr61）, inflammatory factors （TNF-α、IL-6and IL-1β） and restenosis of injured carotid artery. Methods Thirty-six Wistar rats were randomly divided into two groups： model group and control group（n = 18）. The left carotid arteries were injured with a 2F Fogarty balloon catheter to establish the model of carotid artery balloon angioplasty in model group. Specimens were obtained from the injured carotid arteries at different time points （1 d, 4 d, 7 d, 14 d, 28 d and 3 months） , and pathological changes of the arterial wall were observed. The expression of Cyr61、TNF-α、IL-6 and IL-1βin the arterial wall was detected by immunohistochemistry and Real-time PCR technique. The serum concentrations of TNF-α、IL-6 and IL-1β were determined by ELISA. Results Intimal hyperplasia was observed 7 d after balloon injury, and reached the peak on the 28th day. The neointima was not uniform in thickness, and were much thicker than that of the control group （P 〈 0.01 ）. The expression of Cyr61、TNF-α、IL-6and IL-1β increased significantly in the arterial wall of the injured carotid arteries of rats, significantly different from that of the control group （P 〈0.05）. However, no inflammatory factors were detected in serum. Conclusion The expression ofCyr61、TNF-α、IL-6 and IL-1β significantly increases after carotid artery balloon injury in rats, which indicates that Cyt61 and inflammatory factors may play an important role in the vessel wall intimal hyperplasia and vascular restenosis.

关 键 词：富含半胱氨酸61蛋白 炎症因子 颈动脉血管成形术 血管损伤 再狭窄 

分 类 号：R543.5[医药卫生—心血管疾病] R363.21[医药卫生—内科学]