哇巴因对大鼠心室肌起搏通道基因表达的影响  

The Chronic Ouabain Intoxication Upregulates mRNA Expression of Pacemaker Channel in Rat Myocardium

作　　者：贾卫国[1] 邓珏林[1] 黄德嘉[2] 闫乃红[3] 夏庆杰[3] 龙丹[4] 李胜富[4] 

机构地区：[1]四川大学华西医院干部医疗/老年科,四川成都610041 [2]四川大学华西医院心内科,四川成都610041 [3]四川大学华西医院眼科分子生物学研究室,四川成都610041 [4]四川大学华西医院移植免疫研究室,四川成都610041

出　　处：《华西医学》2008年第4期812-814,共3页West China Medical Journal

摘　　要：目的:观察慢性洋地黄中毒对大鼠心室肌起搏通道基因表达的影响。方法:给予大鼠腹腔内注射哇巴因0.045 mg/(kg·d)连续两周。取大鼠心室肌组织,应用半定量实时荧光定量反转录聚合酶链式反应测定起搏通道的基因表达水平。结果:HCN2基因在哇巴因组的表达量较对照组明显升高了近1.5倍(P<0.05),其中以左心室表达量的增加更为明显。而哇巴因组与对照组之间的HCN4基因表达量之间没有统计学差异(P>0.05)。结论:哇巴因慢性中毒时可以导致心室肌的HCN2基因表达明显上调,以左心室为著。而HCN4基因未受影响。HCN2基因可能和洋地黄中毒时出现的自律性增加的心律失常有关。Objective：To observe the influnce of chronic ouabain toxicitation on pacemaker gene expression of rat ventricular myocardium.Methods：An animal model was built successfully for chronic ouabain intoxication with 2 weeks intraperitoneal injection of ouabain at dosage of 0.045 mg/kg per day to rat.The pacemaker gene（HCN）levels were measured using realtime PCR assays.Results：HCN2 mRNA level of ouabain treatment group increased to almost 1.5 times of control group（P〈0.05）.HCN4 mRNA level had no significant change between ouabain group and control group（P〉0.05）.Conclusion：HCN2 mRNA level increases in ouabain-intoxicated myocardium,even more in left ventricle.HCN4 mRNA level does not change with ouabain action.

关 键 词：哇巴因 起搏通道 MRNA表达 

分 类 号：R96[医药卫生—药理学]