干扰素对M2e-HBc疫苗鼻内免疫的佐剂作用  

The Adiuvant effect of Interferon on Intranasal Immunity of M_(2e)-HBc Vaccin

作　　者：吕宏亮 刘松友[2] 董小曼 沈心亮[2] 毕胜利[4] 冯玉萍[5] 马忠仁[5] 

机构地区：[1]北京乾元浩生物股份有限公司,100083 [2]北京生物制品研究所,100024 [3]北京天坛生物制品股份有限公司,100024 [4]中国疾病控制中心病毒病控制所,北京100052 [5]西北民族大学生命科学与工程学院,730030

出　　处：《医学研究杂志》2008年第11期97-99,共3页Journal of Medical Research

摘　　要：目的研究干扰素对鼻腔黏膜免疫M2e-HBc疫苗的佐剂作用。方法小鼠经鼻腔黏膜免疫,用流感病毒攻击。测定抗体水平和对小鼠的保护。结果小鼠可产生局部和系统保护性抗体IgA、IgG,对流感病毒的亚致死性攻击产生完全保护。结论干扰素对M2e-HBc疫苗鼻内免疫具有较强的免疫佐剂作用。Objective To investigate adjuvant effect afforded by type I IFN adjuvanted of intranasally administered universal influenza vaccine M2e - HBc. Methods M2e - HBc vaccine 15ug were formulated with or without IFN as adjuvant and administered three times, at three weeks intervals,by intranasal instillation. All mice were challenged with 4 LD52 of a mouse adapted influenza A virus. Results Mice vaccinated with M2e -HBc adjuvanted IFN, M2e -HBc, control demonstrated high antibody titers to M2e -HBc protein;however,M2e - HBc plus IFN elicited antibody titers that were higher than those induced by either M2e - HBc or IFN alone. Vaccination of mice with M2e - HBc ,IFN adjuvantcd vaccine can protect mice completely from a sub - lethal challenge with influenza A virus. Conclusion Universal Influenza vaccine M2e - HBc adjuvanted IFN is a promising candidates of vaccine against influenza.

关 键 词：M2e-HBc疫苗 鼻内免疫 干扰素 佐剂作用 

分 类 号：R512.91[医药卫生—内科学] R512.620.5[医药卫生—临床医学]