嗅鞘细胞复合于定向性硫酸肝素-胶原蛋白支架移植促进脊髓损伤轴突再生  

Transplantation of olfactory ensheathing cells seeded in linearly oriented nerve guidance scaffolds promotes axon regeneration in spinal cord injury

作　　者：王树森[1] 罗卓荆[1] 韩一生[1] 胡蕴玉[1] 李明全[1] 刘慧玲[2] 吕荣[1] 孟国林[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,陕西西安710033 [2]第四军医大学全军神经科学研究所,陕西西安710033

出　　处：《第四军医大学学报》2008年第21期1923-1927,共5页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30070761);国家教育部高等院校骨干教师资助计划

摘　　要：目的:观测嗅鞘细胞复合于定向性硫酸肝素-胶原蛋白支架材料,对移植修复脊髓损伤的疗效.方法:采用胶原蛋白和硫酸肝素在冷凝条件下制备具有纵行、平行排列微管的硫酸肝素-胶原蛋白基质材料.将嗅鞘细胞与硫酸肝素-胶原蛋白支架材料复合,构建新型神经组织工程材料,移植修复大鼠3mm节段性脊髓损伤.移植术后12wk经神经学观察、电生理学、形态学检测其修复疗效.结果:NF,GAP-43免疫荧光双标和甲苯氨兰染色、电镜观察证实脊髓再生神经纤维成功地长入该神经组织工程材料内.经BBB评分和电生理学检测,部分大鼠运动功能恢复良好.结论:以嗅鞘细胞复合于定向性硫酸肝素-胶原蛋白支架材料,并移植修复脊髓损伤,可以促进脊髓轴突的再生.AIM： To observe the effect of transplantation ofolfactory ensheathing ceils seeded in directional collagen-hepafin sulfate scaffold on spinal cord injury repairing. METHODS： Collagen-heparin sulfate scaffold with longitudinal, paralleled microtubules, was produced under freeze-drying condition. Olfactory ensheathing cells and this scaffold was compounded together to construct new type of nervous tissue engineering material, which was transplanted to repair 3 mm segmental rat spinal cord injury. Neurological observation, eleetrophysiological and morphological detection were performed 12 weeks after transplantation to explore the repairing effect. RESULTS： NF and GAP-43 double-labeled immunofluorescenee, MBP immunofluorescence, toluidine staining and eletron-mieroscope observation all proved that the regenerated spinal nerve fiber successfully grew into this nervous tissue engineering material. BBB scoring and electrophysiologieal detection proved that part rats reaequired satisfactory motor function. CONCLUSION： Collagen-heparin sulfate scaffold compounded with olfactory ensheathing eells could be transplanted to repair spinal cord injury, which could promote the spinal axon regeneration.

关 键 词：脊髓损伤 嗅鞘细胞 硫酸肝素 胶原蛋白 神经组织工程 轴突再生 

分 类 号：R651.2[医药卫生—外科学]