DNA分子原位杂交(in situ hybridization)在植物分子细胞遗传学研究中的应用  

Application of in situ Hybridization in Plant Molecular Cytogenetics Research

作　　者：别同德 张伯桥 高德荣 程顺和 马谈斌 

机构地区：[1]扬州农科院国家小麦改良分中心,江苏扬州225007

出　　处：《中国农学通报》2008年第11期85-92,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"小片段小麦异易位系的高效诱致和精确鉴定"(30270827);国家863计"划抗病;优质;高产小麦新品种分子设计育种"(2006AA10Z1F6);国家科技支撑计划项目"优质高产专用小麦育种技术研究及新品种培育"(2006BAD01A02-19)。

摘　　要：DNA分子原位杂交(in situ hybridization,ISH)是植物分子细胞遗传学研究的重要工具。简要回顾了DNA分子原位杂交的起源和发展,详细综述了基因组原位杂交(genomic in situ hybridization,GISH)在植物细胞遗传学研究中的应用以及荧光原位杂交(Fluorescence in situ hybridization,FISH)在植物物理作图和染色体识别中的应用。还介绍了Fiber-FISH、BAC-FISH以及Immuno-FISH等新兴技术,最后对FISH技术进行了展望。In situ hybridization is one of the most important techniques in plant molecular cytogenetics research. In this paper, we briefly introduced the origin and development of in situ hybridization, firstly. Then, application of genomic in situ hybridization （GISH） in plant cytogenetics research as well as fluorescence in situ hybridization （FISH） in plant physical mapping and chromosome identification were detailed reviewed. Some new techniques such as fiber-FISH, BAC-FISH, and immuno-FISH were also introduced in this pa- per. And a prospect about FISH was given out.

关 键 词：FISH GISH ISH 分子细胞遗传学 植物 

分 类 号：Q343.22[生物学—遗传学]