口蹄疫146S病毒粒子抗原对微血管内皮细胞分泌IL-1的影响  

Effect of Foot-and-Mouth Disease Virus 146S Antigen on IL-1 Production by Microvascular Endothelial Cells

作　　者：张涛[1,2] 田丽芳 张爽[1] 胡格[2] 张涛[2] 穆祥[2] 滕可导[1] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]兽医学(中医药)北京市重点实验室 [3]北京农学院动物科学技术系,北京102206

出　　处：《中国畜牧兽医》2008年第11期62-65,共4页China Animal Husbandry & Veterinary Medicine

基　　金：北京市自然科学基金(6021001);北京市科技新星项目(2006A26)

摘　　要：白介素1(IL-1)是免疫与炎症反应启动的重要调节因子。为了研究微血管内皮细胞(microvascular endothelialcells,MVECs)在口蹄疫病毒(foot-and-mouth disease virus,FMDV)免疫反应中的作用,本试验采用ELISA方法检测了FM-DV 146S抗原对体外培养大鼠心肌膜MVECs分泌IL-1α和IL-1β的影响。研究结果发现,正常情况下大鼠心肌膜MVECs能够低水平的分泌IL-1α和IL-1β,FMDV 146S刺激后其分泌量显著增加。结果表明,MVECs通过上调IL-1分泌,在FMDV抗原的免疫反应中发挥重要的调控作用。IL-1 plays a key regulatory role in the onset of immune and inflammatory responses. To investigate the role of microvascular endothelial cells （MVECs） in the immune responses to foot-and-mouth disease virus （FMDV）, the effects of FMDV 146S antigen on IL-1α and IL-1β production by rat myocardium microvascular endothelial cells （RMMVECs） were assayed by ELISA kit. The study showed that RMMVECs constitutively secreted IL-1α and IL-1β in low level, and their production were significantly upregulated by the stimulation of FMDV 146S antigen. The results indicate that MVECs play an important role in regulating the immune response to FMDV antigen by enhanced production of IL-1.

关 键 词：口蹄疫146S病毒粒子 微血管内皮细胞 白介素-1Α 白介素-1Β 

分 类 号：S852.33[农业科学—基础兽医学]